| Detection of toxigenic Clostridium difficile by polymerase chain reaction |
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| 作者姓名： | 陈村龙 周殿元 潘令嘉 王继德 邓惠欢 |
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| 作者单位： | Department of Gastroenterology，Nanfang Hospital，First Military Medical University，Guangzhau 510515 |
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| 摘 要： | ＤｅｔｅｃｔｉｏｎｏｆｔｏｘｉｇｅｎｉｃＣｌｏｓｔｒｉｄｉｕｍｄｉｆｆｉｃｉｌｅｂｙｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎＣｈｅｎＣｕｎｌｏｎｇ（陈村龙）；ＺｈｏｕＤｉａｎｙｕａｎ（周殿元）；ＰａｎＬｉｎｇｊｉａ（潘令嘉）；ＷａｎｇＪｉｄｅ（...
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Detection of toxigenic Clostridium difficile by polymerase chain reaction |
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| Chen Cunlong, Zhou Dianyuan, Pan Lingjia,Wang Jide, Deng Huihuan.Detection of toxigenic Clostridium difficile by polymerase chain reaction[J].Journal of Medical Colleges of PLA(China),1994(1). |
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| Authors: | Chen Cunlong Zhou Dianyuan Pan Lingjia Wang Jide Deng Huihuan |
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| Abstract: | The polymerase chain reaction (PCR) was used for the detection of toxigenic Clostridium difficile (Cd). A primer pair derived from non-repeating sequences of the toxin A gene were used to amplify a 306 bp DNA fragment. Amplified products were visualized by polyacrylamide gel electrophoresis followed by ethidium bromide staining. All 19 strains of toxigenic Cd generated single specific amplified DNA. In contrast, none of the 8 strains of non-toxigenic Cd, 2 strains of C. sordelli and 2 strains of E. coli gave positive results. After the detected DNA of toxigenic Cd was diluted to 0.5ng, the polymerase chain reaction assays were still positive. The results demonstrate that polymerase chain reaction is a simple, rapid, specific and sensitive method for the detection of toxigenic Cd and could be used for the direct detection of Cd in feces samples. |
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| Keywords: | polymerase chain reaction toxigenic Clostridium difficile |
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