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Compositional sugar analysis of antitumor polysaccharides by high performance liquid chromatography and gas chromatography |
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| Authors: | Yeong Shik Kim Kyung Shin Park Ho Koon Park Sung Whan Kim |
| Affiliation: | 1. Natural Products Research Institute, Seoul National University, 28 Yeonkun-Dong, Jongno-Ku, 110-460, Seoul, Korea 2. Korea Institute of Science and Technology, 136-714, Seoul, Korea 3. National Institute of Health, 122-020, Seoul, Korea |
| Abstract: | Carbohydrate analysis is important in studying structure and activity of complex polysaccharides. New analytical method was applied to get an information on the composition of polysaccharides showing antitumor activity. Monosaccharides were labeled with 7-amino-1,3-naphthalenedisulfonic acid (7-AGA) by reductive amination and separated by HPLC. Five kinds of polysaccharides fromBasidiomycetes were hydrolyzed and analyzed in combination with electrophoresis and HPLC. At the same time, alditol acetate derivatives were prepared and analyzed by gas chromatography. Two different techniques using different derivatization methods showed very similiar results. The monosaccharides fromCoriolus versicolor andLentinus eodes were mainly composed of D-glucose, while those fromGanoderma lucidium andCordyceps militaris were glucose and galactose.Phellinus linteus composed of glucose, galactose, mannose, arabinose and fucose. The HPLC method with fluorescence detector was very sensitive compared to other methods. |
| Keywords: | Carbohydrate analysis Reductive amination HPLC GC |
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