p，p＇-DDE、β-BHC对大鼠支持细胞FasL mRNA表达及NF-κB活性的影响

目的]研究有机氯农药DDT和六六六的代谢产物（p，p＇-DDE）和β-BHC及联合染毒对离体培养大鼠支持细胞（sertoli cell）FasL mRNA表达及NF—κB活性的影响。方法]分离大鼠睾丸支持细胞进行原代培养2d，以DMSO为溶剂对照，分别以终浓度为10、30、50μmol／L的p，p＇-DDE、β-BHC及联合染毒支持细胞24h，应用RT-PCR法研究支持细胞FasL mRNA的表达；用激光共聚焦显微镜检测NF—κB的转位激活状况。结果]10、30、50μmol／L的p，p＇-DDE、β-BHC及其联合染毒支持细胞后，FasL mRNA表达升高，50μmol／L剂量组与对照组差异有统计学意义（P〈0．05），各组内低、中剂量组与高剂量组差异有统计学意义（P〈0．05）；NF-κB活性明显增强。结论]支持细胞经p，p＇-DDE、β-BHC及其联合染毒后，FasL mRNA表达明显升高，NF—κB的活性随染毒剂量的增加而增强，且联合作用比单独作用更加明显。

Combined Effects of p,p'-DDE and β-BHC on the Expression of FasL mRNA and Activation of NF-κB in Rat Sertoli Cells

Objective ] To study the combined effects of p, p＇-DDE and β-BHC on the expression of FasL mRNA and activation of NF-κB of rat Sertoli cell in vitro. Methods ] Sertoli ceils after separating from testicular tissue of SD rats were cultured for 2d, then incubated with DMSO as control group, also incubated with p, p＇-DDE, β-BHC and their combination as test groups in a series of concentrations （ 10, 30, 50 μmol/L ） respectively in vitro for 24h. The FasLmRNA expression was detected by two-step RT-PCR; NF-κB activation state was examined under laser confocus microscope. Results ] The expression of FasL mRNA was increased with the increasing of toxicant concentrations in cell culture. There was statistical significance between the concentrations of 50μmol/L and control group separately （ P 〈 0.05 ）, there was also statistical significance between the concentrations of 50 μmol/L and 10, 30 μmol/L separately （P 〈 0.05 ）. The transposition of NF-κB was obviously increased. Conclusion ] The results suggest that the expression of FasL mRNA can be enhanced by p, p＇-DDE, β-BHC and their combination, the activation of NF-κB can be enhanced with the increase of concentration of p, p＇-DDE, β-BHC and their combination. The combined effects of p, p＇-DDE and β-BHC on Sertoli ceils were more obvious.