釉基质蛋白和骨形成蛋白对牙周膜成纤维细胞增殖活性的影响

目的:通过检测釉基质蛋白(Enamel matrix proteins,EMPs)、骨形成蛋白-2(BMP-2)作用下对促进牙周膜成纤维细胞增殖活性的影响,探讨EMPs、BMP-2促进牙周组织再生的作用机制及其之间的相互影响.方法:酶消化法获得人牙周膜成纤维细胞(Haman periodontal ligament cells,HPDLC)用不同浓度的因子进行诱导,在3d、7d两个时间点,利用MTT法检测和分析诱导后细胞增殖活性的变化.结果:BMP-2、EMPs单独应用均可促进人PDLCs增殖,BMP-2作用强于EMPs.而联合组与100～200μg/L BMP-2、200 mg/L EMPs无明显差异,与100 mg/L EMPs差异显著.结论:BMP-2、EMPs单独或联合应用均可促进人PDLCs增殖,BMP-2作用强于EMPs.联合组中起主要作用的是BMP-2,EMPs未能增加BMP-2的作用.

The effects of enamel matrix proteins and bone morphogenetic protein 2 on cell proliferation by cultured human periodontal ligament cells

Objective To investigate the effects of enamel matrix proteins and bone morphogenetic protein 2 on cell proliferation by cultured human periodontal ligament cells.Methods HPDLCs were dissected and cultured by digestion with bacterial collagenase.Then HPDLCs were treated with different concentration of EMPs and BMP-2.MTT method was used to determine the change of proliferative activity.Results Both BMP-2 and EMPs promoted the ability of cell proliferative activity.BMP-2 was better than EMPs.Distinctness difference was in combined group and 100mg/L EMPs group;and unconspicuous difference was in BMP-2 groups and 200mg/L EMPs group and combined group.Conclusions Both BMP-2 and EMPs could increase the proliferative activity of HPDLCs.BMP-2 was better than EMPs.In combined group,BMP-2 play a main activity.