孕鼠铅暴露对仔鼠肝、脑组织氧化损伤的影响

目的 探讨宫内铅暴露对仔鼠肝、脑发育及其氧化损伤的影响。方法 采用灌胃方式于C57小鼠孕第0天至妊娠结束进行醋酸铅染毒，仔鼠出生后第1天称窝重、肝及脑湿重，测定肝、脑组织丙二醛(MDA)、还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活性，同时取母鼠全血测定血铅含量。结果 宫内铅暴露降低仔鼠窝平均体重及肝、脑湿重随染铅剂量的增加，仔鼠肝、脑组织MDA及还原型GSH含量升高，SOD活性降低，均呈明显的剂量—效应关系。结论 宫内铅暴露对新生仔鼠肝、脑组织损伤与成年及婴儿期铅暴露的损伤机制一致，即铅可能通过干扰过氧化和抗氧化系统的平衡，导致发育期肝、脑组织的损伤。

Effect of lead exposure during pregnancy on oxidation stress in the liver and brain of mice offspring

Objective To investigate the influence of lead exposure during pregnancy on the liver and brain development and oxidative stress in the offspring tissues. Methods The C 57 mice from the day 0 to the end of gestation were treated with lead acetate by gavage. The litter weights and liver, brain weights were quantified after the mice were born. The levels of MDA, GSH and the SOD activities in the newborn mice liver and brain tissues were determined. The content of blood lead of the pregnant mice was also determined. Results The liver, brain and average nest weights appeared to be reduced in the pregnant mice exposed to lead. With the increasing of lead exposure, the level of MDA and GSH in tissues elevated while the SOD activity decreased. The changes showed a dose-dependent correlation with the dosage of lead exposure. Conclusion There was the same mechanism of the influence of lead on the embryo of adult. The influence of lead on the developing liver and brain might be due to its disturbance to the homeostasis of oxidation and anti-oxidation system.