小干扰RNA抑制c-Myc表达对MCF-7细胞增殖和凋亡的影响

目的应用小干扰RNA（siRNA）干扰乳腺癌MCF-7细胞的c-Myc表达，了解c-Myc对肿瘤细胞增殖和凋亡的作用。方法将c-Mye siRNA，经IApofectamineTM2000脂质体转染MCF-7细胞后，实时定量RT-PCR和Western Blotting法检测细胞cMyc mRNA和蛋白的表达，MTT法和流式细胞仪检测干扰后细胞的增殖和凋亡水平。结果转染后MCF-7细胞的c-Myc的mRNA与蛋白表达明显减弱，增殖能力显著降低。洲亡率明显提高。结论运用RNAi技术，可以有效地干扰MCF-7细胞c—Myc的表达，抑制细胞增殖，并诱导细胞渊亡。

Inhibition of Knockdown of c-Myc Expression by siRNA on MCF-7 Breast Cancer Cells Growth and Induction of the Cells Apoptosis

Objective To evaluate the effect of small interferring RNA(siRNA) targeting c-Myc gene on the proliferation and apoptosis of MCF-7cells.Methods SiRNA for c-Myc gene was transfected into MCF-7 cells by LipofectimineTM2000,c-Myc gene expression was assessed by real-time quantitive PCR,and c-Myc protein expression was tested by Western Blotting.MTT assay was used to assess the proliferation of the cells.Fluorescence-activated cell sorter analysis was used to determine apoptosis of the cells.Results Our data showed that the expression of c-Myc gene in MCF-7 cells can be effectively suppressed by siRNA,and the growth rate of MCF-7 cells is decreased markedly.We also found that depeletion of c-Myc in this matter promoted apoptosis of MCF-7 cells.Conclusion The expression of c-Myc in MCF-7 cells can be decreased by RNAi,which can inhibit MCF-7 cells growth and promote the apoptosis of the cells.