左旋精氨酸甲酯对脑缺血再灌注期脑组织炎症反应的影响

目的:探讨局灶性脑缺血再灌注早期应用左旋精氨酸甲酯(L-NAME)对脑组织炎症反应的影响。方法:采用大脑中动脉线栓/再灌注模型,设定对照组、再灌注组、生理盐水(NS)组及L-NAME组,取缺血区组织检测髓过氧化物酶(MPO)活性,做细胞间黏附分子-1(ICAM-1)免疫组化染色,光镜下计数ICAM-1阳性血管数。结果:L-NAME组ICAM-1阳性血管数明显少于再灌注组及NS组(P<0.01)。L-NAME组MPO活性明显低于再灌注组及NS组(P<0.01)。结论:再灌注早期应用L-NAME能明显减少缺血区中性粒细胞浸润及ICAM-1的表达,有助于改善炎症反应导致的再灌注损伤。

Effect of L-NAME on Brain Inflammation during Focal Cerebral Ischemia Reperfusion in Rats

Objective To study the effect of L-NAME on brain inflammatory response during focal cerebral ischemia reperfusion in rats.Methods Focal cerebral ischemia was induced in anesthetized rats by using a middle cerebral artery occlusion(MCAO) model.Twenty-eight male Sprague Dawley rats were randomly divided into four groups:sham group,reperfusion group,physiological saline group and L-NAME group.Myeloperoxidase(MPO) activity assay and calculation of blood vessels with positive immunohistochemical staining of intercellular adhesion molecule-1(ICAM-1) were performed in the ischemic brain tissues.Results The ICAM-1-positive blood vessels in L-NAME group were significantly fewer than those in reperfusion group and physiological saline group at 24 h of reperfusion after 1 h of ischemia(P<0.01) .The MPO activity in L-NAME group was significantly lower than that in reperfusion group and physiological saline group(P<0.01) .Conclusion L-NAME could evidently decrease neutrophil infiltration and ICAM-1 expression in the ischemic areas.It might be helpful to ameliorate the reperfusion injury induced by brain inflammatory response.