| Effects of Serum from Aplastic Anemia patients on the Expression of Cyclin D3 Isoform in Umbilical Cord Blood CD34~ Cells |
| |
| 作者姓名： | 孟凡凯 谭细友 刘文励 孙汉英 周剑锋 李春蕊 刘丹 何莉 孙岚 |
| |
| 作者单位： | Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Hematology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China |
| |
| 基金项目： | ThisprojectwassupportedbyagrantfromNationalNaturalSciencesFoundationofChina (No .30 0 70 32 6 ) . |
| |
| 摘 要： | Aplasticanemia (AA )isabonemarrowfailuresyndrome .Recentstudiesindicatethatimmune me diatedsuppressionofhematopoiesisplaysanimpor tantroleinmostcasesofAA ,namely ,abnormallyactivatedTcellsand/orsomesolublecytokinesinhibitproliferationofhematopoieticstem/p…
|
Effects of Serum from Aplastic Anemia patients on the Expression of Cyclin D3 Isoform in Umbilical Cord Blood CD34~ Cells |
| |
| MENG Fankai,TAN Xiyou,LIU Wenli,SUN Hanying,ZHOU Jianfeng,LI Chunrui,LIU Dan,HE Li,SUN Lan.Effects of Serum from Aplastic Anemia patients on the Expression of Cyclin D3 Isoform in Umbilical Cord Blood CD34~ Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2004(3). |
| |
| Authors: | MENG Fankai TAN Xiyou LIU Wenli SUN Hanying ZHOU Jianfeng LI Chunrui LIU Dan HE Li SUN Lan |
| |
| Institution: | MENG Fankai,TAN Xiyou,LIU Wenli,SUN Hanying,ZHOU Jianfeng,LI Chunrui,LIU Dan,HE Li,SUN Lan Department of Hematology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| |
| Abstract: | Summary: The pathogenesis of aplastic anemia (AA) was explored and the effects of AA serum on the expression of crucial cyclin D isoform (cyclin D3) in umbilical cord blood hematopoietic stem/progenitor cells were observed. The CD34 cells were isolated from the cord blood with MIDI-MACS Semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; The expression level of cyclin D3 was assayed by semi-quantitative RT-PCR and Western-blot after the hematopoietic stem/progenitor cells were incubated in AA serum. The results showed that the AA serum could inhibit the formation of CFU-GM and down regulate the expression level of the cyclin D3 at the mRNA and protein level respectively. In conclusion, the AA serum could inhibit the proliferation of hematopoietic stem cells and down regulate level of cyclin D3, which might be one mechanism of hematopoiesis inhibition in AA. |
| |
| Keywords: | aplastic anemia hematopoiesis stem/progenitor cells cyclin D3 |
| 本文献已被 CNKI 等数据库收录！ |
