| 人牙髓细胞原代培养方法的比较研究 |
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| 引用本文： | 卢婧,唐荣银,李彦.人牙髓细胞原代培养方法的比较研究[J].牙体牙髓牙周病学杂志,2006,16(6):311-313. |
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| 作者姓名： | 卢婧 唐荣银 李彦 |
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| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
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| 摘 要： | 目的：比较不间的人牙髓细胞原代培养方法，优化培养条件，提高培养成功率。方法：分别采用组织块法、酶消化法、组织块酶消化法进行人牙髓细胞原代培养。结果：组织块酶消化法中组织块解离、贴附和细胞游出状况均优于单纯组织块法或单纯酶消化法，成功率较高。结论：采用组织块酶消化法可提高人牙髓细胞原代培养的成功率。
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| 关 键 词： | 人牙髓细胞 体外培养 胎牛血清 |
| 文章编号： | 1005-2593（2006）06-0311-03 |
| 收稿时间： | 2006-04-12 |
| 修稿时间： | 2006-05-16 |
Study of primary culture method of human pulp cell |
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| LU Jing,TANG Rong-yin,LI Yan.Study of primary culture method of human pulp cell[J].Chinese Journal of Conservative Dentistry,2006,16(6):311-313. |
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| Authors: | LU Jing TANG Rong-yin LI Yan |
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| Abstract: | AIM:To compare the primary culture methods of human pulp cells,to enhance the culture condition,and give rise of the success rate of cultivation.METHOD: Human pulp cells were primary cultured with tissue block method,enzymolytic method,and tissue block enzymolytic method separately.RESULTS: The decollation and coherence of the tissue block and the cell growth rate of the tissue block enzymolytic group are better than that of the tissue block group and enzymolyfic group.CONCLUSSION: The tissue block enzymolytic method can elevate the success rate of primary cultivation of human pulp cells. |
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| Keywords: | human pulp cell in vitro culture fetal bovine serum |
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