旋毛虫肌蚴可溶性抗原与日本血吸虫病患者血清的交叉反应

应用ＥＬＩＢ技术观察旋毛虫肌蚴抗原（ＴｓＭＬＳＡ）与６种寄生虫病人血清的交叉反应，结果表明：ＴｓＭＬＳＡ中３１～１００ＫＤａ，内的蛋白大部分被急性血吸虫颊人血清所识别，而慢性血吸虫病人血清仅识别其中的４４／４５，５１／５３，６２／６４及１００ＫＤａ蛋白，小于６０ＫＤａ者可与丝虫病，钩虫病，蛔虫病和肝吸虫病人血清呈不同程度的交叉反应，以前三者为最明显，４５ＫＤａ蛋白可被部分正常人血清识别，提示，旋毛

Cross reaction of Trichinella spiralis larva antigens with sera of schistosomiasis japonica]

Cross reactions of T. spiralis muscular larva antigens (TsMLA) against several sera from patients with other parasitic diseases by ELIB (enzyme-linked immunoblotting technique) were studied. The proteins of 31-100 KDa were recognized by sera of two cases of acute schistosomiasis, while proteins of 44/45, 51/53, 62/64 and 100 KDa were recognized by sera of 11 cases of chronic schistosomiasis. The proteins of 60 KDa showed varied cross reactions against sera of five kinds of patients with other parasitic diseases (filariasis, hookworm diseases, paragonimiasis, ascariasis and clonorchiasis). Only the proteins of 45 KDa were recognized by six of fifteen cases of normal human sera. All of > 29 KDa proteins were recognized by sera of rabbit infected by T. spiralis. It is suggested that there were many antigens in Trichinellen muscular larva which shared in common with several other parasites. The cross reactions of TsMLA against Schistosoma japonicum may be differentiated on the positive reaction bands of 44/45, 51/53, 62/64 and 100 KDa proteins which was specific to the chronic schistosomiasis using the TsMLA by means of ELIB.