茶多酚诱导人胃癌细胞凋亡

为寻找新的肿瘤细胞凋亡诱导剂，选用茶的主要活性成分茶多酚，采用噻唑蓝（ＭＴＴ）还原法，ＤＮＡ凝胶电流透射电镜技术，观察茶多酚对人胃癌细胞（ＭＧＣ－８０３）凋亡的影响。结果表明，被１２５μｇ．ｍｌ＾－１茶多酚２４ｈ后，ＭＧＣ细胞进行凝胶电泳呈现出典型的ＤＮＡ条带，透射电镜下看到凋亡小体；茶多酚对ＭＧＣ细胞凋亡的诱导活性平行于它的细胞毒作用。

Induction of apoptosis by tea polyphenols in MGC cells]

In order to search for tumor cells apoptosis inducer, the apoptosis effects and mechanism of tea polyphenols were studied. Tea polyphenols is an active compounds purified from tea. The apoptosis effects of tea polyphenols were observed on the human gastric carcinoma cells (MGC) by MTT reduction test, DNA agarose gel electrophoresis and transmission electron microscopy (TEM) technique. Having been treated by tea polyphenols in 125 micrograms.ml-1 for 24 h, DNA extracted from MGC cells showed a typical internuclesosomal DNA degradation, i.e., DNA ladder and apoptotic vehicles were observed under TEM. The effects of tea polyphenols were shown to parallel with its cytotoxic activity in MGC cells. These results suggest that the mechanism of antitumor action of tea polyphenols is related to its apoptosis inducing activity.