| 应用原位多聚酶链反应检测喉癌组织中p53基因 |
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| 引用本文： | 谢民强. 应用原位多聚酶链反应检测喉癌组织中p53基因[J]. 湖南医科大学学报, 1997, 22(5): 459-461 |
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| 作者姓名： | 谢民强 |
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| 摘 要： | 采用原位多聚酶链反应技术检测了５例喉癌石蜡组织切片中ｐ５３基因，结果在全部标本中９０％以上的癌细胞检测到ｐ５３基因，平均拷贝数为２．２４±０．２３。表明该技术可用于单拷贝基因研究，并对其改进和应用进行讨论。
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| 关 键 词： | 喉肿瘤 多聚酶链反应 p53基因 |
Study of detecting p53 gene of laryngeal carcinoma on paraffin-embedded tissue sections by in situ polymerase chain reaction] |
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| M Xie. Study of detecting p53 gene of laryngeal carcinoma on paraffin-embedded tissue sections by in situ polymerase chain reaction][J]. Bulletin of Hunan Medical University, 1997, 22(5): 459-461 |
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| Authors: | M Xie |
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| Affiliation: | Department of Otolaryngology, Xiangya Hospital, Hunan Medical University, Changsha. |
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| Abstract: | In order to investigate alterations of p53 gene in laryngeal carcinoma, five cases of paraffin-embedded tissue were detected by in situ polymerase chain reaction (PCR). The results showed that p53 gene was presented in more than 90% of cancer cells in all cases with exon 5 specific primers, which signal copy numbers were 2.24 +/- 0.23. It suggests that in situ PCR and paraffin-embedded tissue are able to be used in the single copy gene research. The improvement and application of the technique were discussed. |
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