组织工程化肌腱对T淋巴细胞亚群及其受体的影响

目的：探讨组织工程化肌腱对T淋巴细胞亚群及受体的影响。明确其组织相容性，方法：采用3月龄法国罗曼鸡75只，随机分成五组，每组15只，手术造成足第3趾屈深肌腱2．5cm缺损后，分组。A组：未手术组，B组：自体肌腱移植组；C组：新鲜同种异体肌腱移植组；D组：梯度降解材料复合腱细胞移植组；E组、；衍生肌腱材料复合腱细胞移植组，检测CD4＋，CD8＋，CD28细胞阳性率及T淋巴细胞相关抗原受体（TCR）密度变化，通过刀豆蛋白（ConA)诱导的淋巴细胞转化试验，直接淋巴细胞混合培养试验及间接淋巴细胞混合培养试验，了解机体对肌腱细胞的免疫反应水平。结果：D，E组与B组比较，早期CD4＋，CD8＋ＴＣＲ轻度增高，２周后下降，无显著性差异（Ｐ＞０．０５），C组CD4＋，CD8＋，CD28及TCR阳性率与D，E组比较，有显著性差异（P＜0．05），结论：肌腱细胞为弱抗原细胞，抗原脱落或可溶性抗原分泌较少，组织工程化肌腱有良好的组织相容性。

THE INFLUENCE OF TISSUE ENGINEERED TENDON ON SUBGROUP OF T LYMPHOCYTES AND ITS RECEPTOR IN ROMAN CHICKENS

OBJECTIVE: To investigate the influence of tissue engineered tendon on subgroup of T lymphocytes and its receptor in Roman chickens. METHODS: The flexor digitorum profundus of the third toes of right feet in 75 Roman chickens were resected and made 2.5 cm defects as experimental model. They were randomly divided into five groups according to five repair methods: no operation (group A), autograft (group B), fresh allograft (group C), polymer combined with allogenous tendon cells (group D), derived tendon materials combined with allogenous tendon cells (group E). The proliferation and transformation of lymphocytes and contribution of CD4+, CD8+, CD28 and T cell receptor (TCR) were detected to study the immune response. RESULTS: The CD4+, CD8+ and TCR of group D and E were increased slightly than that of group B after 7 days, while after 14 days, those data decreased gradually and no significant difference between tissue engineered tendon and autografts (P > 0.05), and there was significant difference between fresh allograft and tissue engineered tendon (P < 0.05). Lymphocytes transformation induced by conA also showed no significant difference between tissue engineered tendon and autografts (P > 0.05). CONCLUSION: Tendon cells are hypoantigen cells, there are less secretion of soluble antigen or antigen chips dropped out from cells. Tissue engineered tendon has excellent biocompatibility.