| 乳腺癌冻对蜡块与冻剩蜡块免疫组织化学染色效果比较 |
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| 引用本文： | 刘华庆,黄琼,胡莉. 乳腺癌冻对蜡块与冻剩蜡块免疫组织化学染色效果比较[J]. 贵州医药, 2011, 35(7): 602-604. DOI: 10.3969.j.ISSN.1000-744X.2011.07.008 |
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| 作者姓名： | 刘华庆 黄琼 胡莉 |
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| 作者单位： | 遵义医学院附属医院病理科,563003 |
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| 摘 要： | 目的 探讨冻对蜡块与冻剩蜡块对免疫组织化学染色是否有影响.方法 用免疫组织化学方法检测60例乳腺癌组织标本冻对蜡块和冻剩蜡块中ER、PR、HER-2、P53、Ki-67、E-cadherin的表达情况.结果 总的阳性率无差别,但冻对蜡块的非特异性染色要强于冻剩蜡块.结论 尽量选择冻剩蜡块做免疫组织化学染色;若只有冻对蜡...
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| 关 键 词： | 乳腺癌 冻对蜡块 冻剩蜡块 免疫组织化学 |
Comparison of Immunohistochemical Staining effect in paraffin-imbedded tissues from frozen and unfrozen remnant of Breast cancer specimen |
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| Liu Huaqing,Huang Qiong,Hu Li. Comparison of Immunohistochemical Staining effect in paraffin-imbedded tissues from frozen and unfrozen remnant of Breast cancer specimen[J]. Guizhou Medical Journal, 2011, 35(7): 602-604. DOI: 10.3969.j.ISSN.1000-744X.2011.07.008 |
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| Authors: | Liu Huaqing Huang Qiong Hu Li |
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| Affiliation: | .Department of Pathology,Affiliated Hospital of Zunyi Medical College,Zunyi 563003,China |
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| Abstract: | Objective To explore the effect of immunohistochemistry staining in paraffin imbedded tissues from frozen and unfrozen remnant of specimen.Methods Expression of ER,PR,HER-2,P53,Ki-67,E-cadherin was respectively detected in paraffin-imbedded tissues from frozen and unfrozen remnant of 60 cases of Breast cancer specimens.ResultsThere was no significant difference between the positive expression rates in paraffin-imbedded tissues from frozen and unfrozen remnant,but non-specific stain was stronger in the former than that in the latter.Conclusion Immunohistochemistry should be undertaken in unfrozen remnant of tissue specimen as far as possible.Non-specific stain should be eliminated on the case of frozen remnant. |
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| Keywords: | Breast cancer Paraffin-imbedded tissues from frozen remnant Paraffin-imbedded tissues from unfrozen remnant Immunohistochemistry |
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