结核病患者血清中Small RNAs表达谱分析

目的 了解结核病患者血清中Small RNA(sRNA)表达谱的特征，为从Small RNA角度探讨结核病发生、发展的调控机制奠定基础。 方法 采用Solexa深度测序技术得到包括miRNA、siRNA、piRNA、rRNA、tRNA、snRNA、snoRNA、repeat associate sRNA、exon或intron降解片段等在内的所有Small RNA。通过与已知数据库进行比对、寻找样品与数据库之间在基因组位置上的overlap等方法，对sRNA进行注释，同时选取没有被注释上的sRNA，使用华大自主开发的软件Mireap预测novel miRNA。 结果非结核病组血清中发现109 630种、2 759 795条Small RNA，其中候选miRNA种数为84、候选miRNA总表达量为1 433；结核病组血清中发现97 614种、1 452 119条Small RNA，其中候选miRNA种数为65、候选miRNA总表达量为755。 结论 结核病人与非结核病人血清中的Small RNA表达谱存在种类与数量上的差异。

Analysis of the expression profile for serum small RNAs from tuberculosis patients

Objective To analyze the expression profile of serum small RNAs(sRNA) from tuberculosis(TB) patients,and find a new way to study the regulatory mechanisms in the process of tuberculosis. Methods Small RNAs from Solexa deep sequencing covered almost every kind of RNA,including miRNA,siRNA,piRNA,rRNA,tRNA,snRNA,snoRNA,repeat associated sRNA and degraded tags of exon or intron.By comparing our sequences with those in databases and picking out the overlap on genome location between our data and the databases,sR...