胶束电动毛细管色谱法同时测定人血清中3种抗癫痫药的浓度

目的:建立以胶束电动毛细管色谱法同时测定人血清中苯巴比妥(PB)、苯妥英钠(PHT)和卡马西平(CBZ)浓度的方法。方法:内标为巴比妥,缓冲液为10mmol.L-1磷酸氢二钠-30mmol.L-1十二烷基硫酸钠(胶束相)-15%甲醇,电泳电压为18kV,检测波长为210nm,毛细管柱为弹性石英毛细管柱(37cm×50μm)。结果:PB和PHT线性范围为2.5~80μg.mL-1,最低检测限为0.1μg.mL-1,CBZ线性范围为1~32μg.mL-1,最低检测限为0.2μg.mL-1;PB、PHT、CBZ平均回收率分别为99.90%、99.91%、99.42%。结论:本法快速、简便、准确、灵敏、重现性好,适于临床血药浓度监测和药动学研究。

Determination of Serum Concentrations of Phenbarbital,Phenytoin Sodium and Carbamazepine by MEKC

OBJECTIVE：To determine serum concentrations of phenbarbital（PB） ,phenytoin sodium（PHT） ,carbamazepine （CBZ） by micellar electrokinetic capillary chromatograph（MEKC） .METHODS： The determination was performed on elastic quartz column（37 cm × 50 μm） with barbital as internal standard.The buffer solution consisted of 10 mmol · L -1 phosphate sodium buffer - 30 mmol· L^-1 SDS sodium（micellar phase） - 15% methanol at a constant voltage of 18 kV.The detection wavelength was set at 210 nm.RESULTS： The linear concentration ranges of PB and PHT were 2.5-80 μg· mL^-1, with detective concentration of 0.1 μg · mL^-1; the linear range and detective concentration of CBZ were 1-32 μg· mL^-1 and 0.2 μg ·mL^-1, respectively .The average recoveries of the PB, PHT, and CBZ were 99.90%, 99.91%, and 99.42%, respectively. CONCLUSION ： The method is rapid, simple, accurate, sensitive and reproducible, and suitable for clinical monitoring of drug level and pharmaceutical study.