自身免疫性甲状腺病TSH受体抗体异质性研究

目的 研究自身免疫性甲状腺病（ＡＩＴＤ）患者ＴＳＨ受体抗体（ＴＲＡｂ）的异质性。方法 选择异质性ＴＲＡｂ患者６例，用ＥＢ病毒转染其外周血Ｂ淋巴细胞并筛选能产一单克隆ＴＲＡｂ的阳性细胞孔。结果６例患者的ＴＳＨ结合抑制免疫球蛋白（ＴＢＨⅡ）测定值为５１￣８９％，患者１、２、４、５、６存在甲状腺兴奋性抗体（ＴＳＡｂ），而患者１、２、３可测各甲状腺阻断抗性体（ＴＳＢＡｂ）、以ＥＢ病毒转染外周血Ｂ淋巴细胞后

Study on the Heterogeneity of TSH Receptor Antibodiesin the Pathogenesis of Autoimmune Thyroid Disease

Objective To investigate the heterogeneity of TSH receptor antibodies inpatients with autoimmune thyroid disease(AITD). Methods Six patients with the presenceof heterogeneous TSH receptor antibodies were selected. The EBV - transformed B cellclones producing monoclonal antithyrotropin receptor antibodies were then established fromthe selected patients. Results Total 6 patients with heterogeneous TSH receptor antibody(TRAb) were chosen, with 125I - TSH binding inhibiting immunoglobulin(TBII) values rangingfrom 51% to 89%. Patients No. 1,2,4,5,6 had thyroid stimulating antibody(TSBAb) andpatients No.1,2,3 thyroid stimulating-blocking antibody(TSBAb). After EBV - transformationof those patients' peripheral B lymphocyte, we isolated and characterized five B cell clonesproducing TBII from patient No.3 and four B cell clones producing TSAb from patients No.6.A does - dependent relationship was found between TRAb titers and concentrations of thesupernatant collected from the EBV-- transformed B lymphocytes. Conclusion Autoantibodyof the TSH receptor is a polyclonal one populations heterogeneous with stimulatory andinhibitory effects.