争光霉素A6和它在争光霉素复合物的地位

争光霉素A₅已鉴别为Bleomycin A₆,在争光霉素复合物中所占比例一般在10%左右,在某些批样中可高达15%以上。文献报告Bleomycin A₆在天然产的Bleomyein复合物中只有痕量。通过向发酵培养基中加入特定组分的末端胺可大大提高其特定组分在复合物中的含量比而其它组分的产生则不同程度地被抑制。但Bleomyein A₆例外,即使向培养基中加入其末端胺精胺(0.3mg/ml),在所产生的复合物中大大增多的组分是Bleomyein A₆,而Bleomycin A₆仍只有痕量。这表明争光霉素产生菌有和Bleomycin产生菌明显不同的特点。

ZHENGGUANGMYCIN A6 AND ITS STATUS IN THE COMPLEX OF ZHENGGUANGMYCINS

Based on physico-chemical properties and analysis of spectra, zhengguangmycin A_6 was identified as bleomycin A_6. As reported in literature, the addition of a certain amine to the fermentation media increased the ratio of production of the bleomycin which contains the added amine as the terminal moiety. Spermidine (0.36 mg/ml) Was very efficiently incorporated into bleomyein A_5 and completely suppressed the production of other bleomycins. However, it is an exception for bleomycin A_6, which is present in natural bleomycins in only trace amounts, even after the addition of its terminal amine (spermine 0.30 mg/ml) to the fermentation media. But the amount of zhengguangmycin A_6 is different. Under normal conditions the amount of zhengguangmycin A_6 in natural zhengguangmycins is usually over 10%, in some batches, reaching 15% and was the second important component in an amount next to zhengguangmycin A_5 (pingyangmycin). This suggests that the producing strain of zhengguangmycins, Streptomyces verticillus var. pingyangensis nov. is different from that of bleomycin, S. verticillus.