Neuronal adrenergic and muscular cholinergic contractile hypersensitivity in canine jejunum after extrinsic denervation |
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Authors: | Bruno M Balsiger MD Chong-Liang He MD Nicholas J Zyromski MD Michael G Sarr MD |
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Institution: | (1) Department of Surgery and the Gastroenterology Research Unit, Mayo Clinic, Rochester, Minnesota;(2) Department of Visceral and Transplantation Surgery, University of Bern, Bern, Switzerland;(3) Department of Surgery, Medical College of Ohio, Toledo, Ohio;(4) Gastroenterology Research Unit (AL 2-435), Mayo Clinic, 200 First Street SW, 55905 Rochester, MN |
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Abstract: | Extrinsic denervation may be responsible for motor dysfunction after small bowel transplantation. The aim of this study was
to examine the role of extrinsic innervation of canine jejunum on contractile activity. An in vitro dose response of cholinergic
and adrenergic agonists was evaluated in canine jejunal strips of circular muscle at 0, 2, and 8 weeks in a control group
and after jejunoileal extrinsic denervation (EX DEN). Neurons in circular muscle were quantitated by means of immunohistochemical
techniques. Adrenergic and cholinergic responses did not differ at any time in the control group. However, at 2 and 8 weeks,
extrinsic denervation caused an increased sensitivity to the procontractile effects of the cholinergic agonist bethanechol
at the level of the smooth muscle cells, and increased sensitivity to the inhibitory effects of the adrenergic agent norepinephrine
mediated at the level of the enteric nervous system. Immunohistochemical analysis showed a reduction in all neurons and a
complete lack of adrenergic fibers in the EX DEN group after 2 and 8 weeks. Extrinsic denervation induces enteric neuronal
cholinergic and adrenergic smooth muscle hypersensitivity in canine jejunal circular muscle.
Presented in part at the annual meeting of the American Gastroenterological Association, Orlando, Florida, May 18, 1999 (poster
presentation), and published as an abstract in Gastroenterology 116:A1075, 1999.
Supported by United States Public Health Service grant DK39337 from the National Institutes of Health (M.G.S.); the Swiss
National Science Foundation; the Swiss Society of Gastroenterology and Hepatology; the Swiss Foundation for Medical and Biological
Science; the Novartis Foundation; Astra Zeneca Pharmaceuticals, Switzerland; and the Department of Visceral and Transplantation
Surgery, University of Bern, Switzerland. |
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Keywords: | Motility smooth muscle contractions denervation contractility cholinergic nerves adrenergicnerves denervation hypersensitivity small intestine |
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