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| 高效液相色谱法测定红宝散中欧前胡素的含量 | |
| 引用本文： | 王舒,王苗苗,吕述丽. 高效液相色谱法测定红宝散中欧前胡素的含量[J]. 中国中医药现代远程教育, 2013, 0(8): 149-150 |
| 作者姓名： | 王舒 王苗苗 吕述丽 |
| 作者单位： | 吉林省吉林市食品药品检验所,吉林132001 |
| 摘 要： | 目的采用高效液相色谱法测定红宝散中欧前胡素的含量。方法采用HPLC法，Apollo-C18（250mm×4．6mm，5μm）色谱柱，流动相为甲醇一水（55：45），流速为1．0mL·min-1，紫外检测波长为300nm，柱温为室温。结果欧前胡素进样量在0．0300-0．2000ug线性关系良好，r=0．9999，平均回收率99．68％，RSD=0．35％。结论本方法准确、简便、快速，可作为红宝散中欧前胡素的含量测定。 |
| 关 键 词： | 高效液相色谱法 红宝散 欧前胡素 |
Determination of Imperatorin in Hong Bao powder by HPLC |
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| Wang Shu Wang Miaomiao Lv Shuli. Determination of Imperatorin in Hong Bao powder by HPLC[J]. Chinese Medicine Modern Distance Education of China, 2013, 0(8): 149-150 | |
| Authors: | Wang Shu Wang Miaomiao Lv Shuli |
| Affiliation: | Wang Shu Wang Miaomiao Lv Shuli (Jifin Institute for Food and Dru8 Control, Jifin 132001, China) |
| Abstract: | Objeetive To establish a method for the determination of Imperatorin in Hong Bao powder. Methods HPLC method was used to deter- mine the content. The column was Apollo -C18（250 minX4. 6 ram,5μm） ,and the mobile phase：methanol-water （55 ： 45）. The flow rate was 1.0 mL · rain-1 and the detective wavelength was 300nm. Results The calibration curve of Imperatorin was linear in the range of 0. 0300 0. 2000 μg（ r = 0. 9999）, the average recovery was 99.68 % , RSD = 0. 35 %. Conclusion The method is accurate with good reproducibility and can be used for the content determination of Imperatorin in Hong Bao powder. |
| Keywords: | Hong Bao powder Lmperatorin HPLC |
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