75名不同GSTM1基因型健康大学生鼻腔细菌多样性分析

目的 研究不同GSTM1基因型健康大学生鼻腔菌群的细菌多样性。方法 采集75个健康大学生志愿者鼻腔样品，利用高通量测序分析鼻腔中菌群结构及多样性。结果 调查人群中GSTM1缺失型（GSTM1 null genotype, GN）发生率为53.3%，且GN组个体中的种群丰富度较GSTM1表达型（GSTM1 positive,GP组）个体高。对鼻腔样品细菌群落组成分析发现，优势菌门为厚壁菌门（44.87%）、变形菌门（30.85%）、放线菌门（22.87%），其中女性厚壁菌门的相对丰度(47.63%)高于男性(41.60%)，且在GN组中，女性厚壁菌门的相对丰度(42.37%)远高于男性(28.78%)(P＜0.05)；而放线菌门的相对丰度(20.73%)却低于男性(26.18%) ( P＜0.05)，GP组中，男性梭杆菌门的相对丰度(0.040%)高于女性(0.028%) (P＜0.05)。结论 不同GSTM1基因型人群鼻腔细菌种群构成相似，但种群丰富度有较大差异。性别对不同GSTM1基因型健康成人的微生物分布有影响，为进一步研究大气污染物暴露对不同GSTM1基因型个体的呼吸道健康效应提供了理论依据。

Determination of nasal microbial diversity in 75 healthy college students of different Glutathione S-transferases M (GSTM1) genotypes

Objective To research the nasal community diversity of different genotypes of GSTM1 in healthy college volunteers. Methods Nasal samples of 75 healthy college volunteers were collected and their bacterial biodiversity and community structure were analyzed by the high-throughput sequencing technology. Results The frequency of GSTM1 null genotype(GN) in our volunteers is 53.3%, and the degree of community diversity of GN is higher than the GSTM1 positive genotype(GP) group. The community structure results displayed that dominant proportion phylum include Firmicutes (44.87%), Proteobacteria (30.85%), and Actinobacteria(22.87%). The relative abundance of the Firmicutes in female group (47.63%) was higher than male group(41.60%), especially in GN group, where female had the proportion of 42.37%, which was much higher than male’s 28.78% (P<0.05). Whereas, the opposite trend was shown in Actinobacteria phylum, where female group had the proportion of 20.73%, which was significantly lower than male group(P<0.05). In contrast to Actinobacteria phylum, Fusobacterium phylum was significantly higher in male(0.040%) than in female(0.028%) in GP group(P<0.05). Conclusion The microbial community was similar in different GSTM1 genotypes, but the abundance of community had much difference among different groups. Gender had an effect on the distribution of nasal bacteria in healthy adults between different genotypes, which provided a theoretical basis for further research on the respiratory effects of exposure to air pollutants on individuals with different GSTM1 genotypes.