大气细颗粒物多环芳烃暴露对健康人尿中羟基代谢物和氧化应激水平的影响

目的 研究大气颗粒物（PM2.5）及多环芳烃暴露与人体内羟基代谢物的关系，并探讨其对健康人群氧化应激水平的影响。方法 招募46名在校大学生，采用定组研究的方法，于2015夏季和冬季对招募对象进行2次随访，采集研究对象尿样并测定尿中羟基代谢物和8 - 羟基脱氧鸟苷。结果 PM2.5、∑PAHs浓度和温度在夏季和冬季的差异有统计学意义（P＜0.05）；而相对湿度（RH）间差异无统计学意义；在夏季时，2 - OHFlu、3 - OHPhe、1 - OHPhe和1 - OHPyr浓度依次为0.25、0.16、0.13、0.08 μmol/mol Cr；而冬季浓度依次为0.63、0.73、0.40、0.42 μmol/mol Cr，经配对t检验，2组间差异均有统计学意义。而8 - OHdG虽然在冬季略高于夏季，但2组间差异无统计学意义。经Spearman 相关分析，4种OH - PAHs与∑PAHs均有较强相关性，8 - OHdG、PM2.5、∑PAHs及OH - PAHs无相关性。结论 细颗粒物PM2.5及多环芳烃暴露对人体羟基代谢物水平有影响，冬季羟基代谢物水平高于夏季，而对8 - OHdG的影响不显著。

Effects of fine particulate matter and polycyclic aromatic hydrocarbons exposure on hydroxyl metabolites and oxidative stress levels in urine of healthy adults

Objective To investigate the associations of exposure to ambient particulate matter and polycyclic aromatic hydrocarbons with urinary metabolites of polycyclic aromatic hydrocarbons, and explore the effects of exposure to ambient particulate matter and polycyclic aromatic hydrocarbons on oxidative stress level in healthy adults. Methods A panel of forty-six college students were recruited and followed up twice between summer and winter in 2015. Their urine samples were collected and measured for four mono-hydroxylated polycyclic aromatic hydrocarbons(OH-PAHs) and 8-hydroxy-2′-deoxyguanosine (8-OHdG). Results Significant difference was observed for the concentrations of PAHs, PM2.5 and temperature between summer group and winter group(P<0.05). The concentrations of 2-OHFlu, 3-OHPhe, 1-OHPhe, 1-OHPyr by creatinine adjusted in summer group were 0.25, 0.16, 0.13, 0.08 μmol/molCr, and the concentrations in winter group were 0.63, 0.73, 0.40, 0.42 μmol/molCr, respectively. The difference was significant between summer group and winter group by paired t-test. The levels of 8-OHdG in winter group was higher slightly than summer group, but no significant difference was observed. There was a strong correlation between the four OH-PAHs and ∑PAHs, and no correlation was observed among the 8-OHdG and PM2.5, ∑PAHs, and OH-PAHs by the Spearman correlation analysis. Conclusion These results suggested that exposure to fine particulate matter(PM2.5), especially PAHs, positively correlated with the concentrations of polycyclic aromatic hydrocarbon metabolite. However, the exposure had no effect for 8-OHdG.