张家口地区白细胞介素-28B基因单核苷酸多态性与肝细胞癌的相关性研究

目的 探讨白细胞介素-28B（IL-28B）基因rs12979860位点单核苷酸多态性与张家口居民肝细胞癌遗传易感性的关联及与环境因素间的交互作用。方法 采用以医院为基础1[DK]∶1配比病例对照研究方法，选取肝细胞癌患者218例作为观察组，同期健康体检者218例作为对照组，应用改良多重高温连接酶检测反应技术检测两组IL-28B基因rs12979860位点单核苷酸多态性。采用二分类logistic回归模型分析IL-28B基因rs12979860位点单核苷酸多态性与肝细胞癌遗传易感性的关系，并探究rs12979860位点单核苷酸多态性与环境因素的交互作用对肝细胞癌发病影响。结果 对照组与观察组间IL-28B基因rs12979860位点基因型分布差异无统计学意义（P>0.05）。IL-28B基因rs12979860位点T等位基因可增加肝细胞癌发病风险（P<0.05）。分层分析发现，在饮酒、乙型肝炎病毒（HBV）及丙型肝炎病毒（HCV）感染人群中rs12979860位点TT基因型相比TC+CC基因型，肝细胞癌发病风险升高，差异具有统计学意义（P<0.05）。交互作用分析示，IL-28B基因rs12979860位点单核苷酸多态性与饮酒、HBV及HCV感染均存在正交互作用。结论 在张家口地区，IL-28B基因rs12979860位点单核苷酸多态性与肝细胞癌遗传易感性明显相关，并与饮酒、HBV及HCV感染存在正交互作用，可增加肝细胞癌发病风险。

Correlation between single-nucleotide polymorphisms of interleukin-28B and hepatocellular carcinoma,Zhangjiakou

Objective To investigate the associations between single-nucleotide polymorphisms of IL-28B and the hereditary susceptibility of hepatocellular carcinoma, and their interactions with environmental factors in residents at Zhangjiakou, Hebei. Methods The study was a hospital-based 1:1 matched case-control study. 218 hepatocellular carcinoma patients were enrolled as the hepatocellular carcinoma group, and 218 subjects undergoing a physical examination at the same period were enrolled as the control group. Improved multiple ligase detection reactions was used to detect the genotypes of IL-28B rs12979860. Binary logistic regression analyses were used to explore the associations between single-nucleotide polymorphisms of IL-28B rs12979860 and the hereditary susceptibility of hepatocellular carcinoma, and their interactions with environmental factors. Results No significant difference was observed between the control group and hepatocellular carcinoma group in the distribution of IL-28B rs12979860 genotypes (P>0.05). IL-28B rs12979860 T alley could increase the risk of hepatocellular carcinoma (P<0.05). The stratified analysis found that rs12979860 TT genotype could increase the risk of hepatocellular carcinoma compared to TC + CC genotype (P<0.05) in alcohol, hepatitis B virus (HBV)-infected people, and hepatitis C virus (HCV)-infected people. Gene-environment interaction assay indicated IL-28B rs12979860 SNP positively interacted with alcohol, HBV infection and HCV infection. Conclusion In residents at Zhangjiakou, Hebei, the single-nucleotide polymorphisms of IL-28B rs12979860 was closely related with the hereditary susceptibility of hepatocellular carcinoma, positively interacted with alcohol, HBV infection, and HCV infection, which could increase the risk of hepatocellular carcinoma.