肝星状细胞中Skp2表达及其对纤维化、增值及机制探讨

目的 观察肝星状细胞中Skp2表达及其对纤维化的影响。方法 利用 siRNA 沉默HSC-T6细胞中Skp2基因的表达，CCK - 8 法检测细胞增殖，流式细胞术检测细胞凋亡， qRT - PCR检测Skp2、P27、Col I、Col III mRNA的表达，Western blot方法检测Skp2、P27、Caspase - 3、Survivn的蛋白表达水平。结果 与NC - Skp2组比较，siRNA - Skp2组Skp2 mRNA的相对表达水平显著降低，细胞的增殖能力受抑制，凋亡能力增强（P＜0.05）。此外，与NC - Skp2组比较，siRNA - Skp2组P27 mRNA相对表达水平明显增加，Col I、Col III mRNA相对表达水平明显降低，差异有统计学差异（P＜0.05）；与NC - Skp2组比较，siRNA - Skp2组Skp2、Survivn蛋白水平明显降低，Caspase - 3、P27蛋白水平明显增加，均有统计学差异（P＜0.05）结论 降低Skp2基因在HSC - T6细胞中的表达水平，可抑制细胞的增殖和纤维化。

Expression of Skp2 in hepatic stellate cell and its effect on fibrosis,proliferation and mechanism

Objective To observe the expression of Skp2 in hepatic stellate cells and its effect on fibrosis. Methods siRNA was used to silence the expression of Skp2 in HSC-T6 cells. CCK-8 assay was used to detect cell proliferation. Flow cytometry was used to detect cell apoptosis. qRT-PCR was used to detect the expression of Skp2, P27, Col I and Col III mRNA. Protein expression of Skp2, P27, Caspase-3 and Survivn was detected by Western blot. Results Compared with NC-Skp2 group, the relative expression level of Skp2 mRNA in siRNA-Skp2 group was significantly reduced, cell proliferation was inhibited and apoptosis was enhanced(P<0.05). In addition, compared with NC-Skp2 group, the relative expression level of P27 mRNA in siRNA-Skp2 group was significantly increased, and the relative expression levels of Col I, Col III mRNA were significantly lower(P<0.05); compared with NC- Skp2 group, Skp2, Survivn protein levels in siRNA-Skp2 group decreased significantly, caspase-3, P27 protein levels were significantly increased, there were statistically significant differences(P<0.05). Conclusion Reduce the expression of Skp2 in HSC-T6 cells can inhibit the cells Proliferation and fibrosis.