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| 不同浓度bFGF对犬骨髓基质细胞增殖、分化影响的实验研究 | |
| 引用本文： | 王磊,潘可风,黄远亮,张秀丽.不同浓度bFGF对犬骨髓基质细胞增殖、分化影响的实验研究[J].口腔颌面外科杂志,2008,18(1):6-10. |
| 作者姓名： | 王磊 潘可风 黄远亮 张秀丽 |
| 作者单位： | 1. 同济大学材料科学与工程学院,上海,200092 2. 同济大学附属口腔医学院口腔颌面外科,上海,200072 3. 同济大学附属东方医院口腔科,上海,200120 4. 上海交通大学医学院附属第九人民医院口腔生物工程实验室,上海,200011 |
| 摘 要： | 目的：研究不同浓度的碱性成纤维细胞生长因子（basic fibroblast growth factot,bFGF）对体外培养的beagle犬骨髓基质细胞（bone marrow stromal ceils，BMSCs）增殖和分化的影响。方法：体外培养第2代BMSCs的培养液中分别加入不同浓度（25、50、100、200ng／mL）的bFGF，连续6d倒置显微镜下动态观察BMSCs的形态和生长情况．行MTT比色、碱性磷酸酶（alkalin ephosphatase，ALP）活性定量检测、ALP和Von Kossa染色。结果：浓度为50ng／mL的bFGF可明显促进犬的BMSCs增殖（P〈0．05），但无显著促进犬BMSCs分化的作用（P〉0．051。结论：bFGF能有效促进犬BMSCs的增殖，且与bFGF剂量有关。 |
| 关 键 词： | 骨髓基质细胞 碱性成纤维细胞生长因子 组织工程 种子细胞 |
| 文章编号： | 1005-4979（2008）01-0006-05 |
| 收稿时间： | 2007-10-10 |
| 修稿时间： | 2007年10月10 |
Effects of Basic Fibroblast Growth Factor on the Proliferation and Differentiation of Dog Bone Marrow Stromal Cells |
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| WANG Lei,PAN Ke-feng,HUANG Yuan-liang,ZHANG Xiu-li.Effects of Basic Fibroblast Growth Factor on the Proliferation and Differentiation of Dog Bone Marrow Stromal Cells[J].Chinese Journal of Oral and Maxillofacial Surgery,2008,18(1):6-10. | |
| Authors: | WANG Lei PAN Ke-feng HUANG Yuan-liang ZHANG Xiu-li |
| Institution: | WANG Lei, PAN Ke-feng, HUANG Yuan-liang, ZHANG Xiu-li(1, School of Material Science and Engineering, Tongji University, Shanghai 200092; 2. Department of Oral and Maxillofacial Surgery, School of Stomatology, Tongji University, Shanghai 200072; 3. Department of Stomatology, Oriental Hospital, Tongji University, Shanghai 200120; 4. Lab of Bioengineering, Ninth People＇s Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200011,China) |
| Abstract: | Objective:To investigate the effects of basic fibroblast growth factor(bFGF)on proliferation and differentiation of the beagle dog s bone marrow stromal cells(BMSCs)in vitro.Methods:The 2~(nd)generated BMSC was cultured in vitro with different concentrations(25,50,100,200 ng/mL)of bFGF for 6 days,then detected by MTT,ALP and Von Kossa staining.Results:50 ng/mL bFGF could significantly promote BMSCs proliferation(P<0.05),while not enhance BMSCs differentiation(P>0.05).Conclusion:bFGF is an effective induction factor to regulate proliferation of BMSCs and depending on its proper concentration. |
| Keywords: | bone marrow stromal ceils basic fibroblast growth factor tissue engineering seed cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |