血红素氧合酶-1在胃癌腹膜转移中的表达及意义

目的 探讨血红素氧合酶-1(hemeoxygenase-1,HO-1)在胃腺癌及腹膜转移灶、细胞系及耐药细胞系中的表达及意义.方法 用免疫组化法检测68例胄腺癌组织及其腹膜转移灶、转移灶旁无瘤腹膜组织中HO-1的表达,以及46例无腹膜转移的胃腺癌组织中HO-1的表达.Western印迹法检测胃腺癌腹膜转移组织及耐药细胞系HO-1的表达.结果 胃腺癌及其腹膜转移灶HO-1的阳性表达率分别为39.7%(27/68)和41.2%(28/68),显著高于癌旁无瘤腹膜组织0%(0/68),P<0.01],亦显著高于无腹膜转移胃癌组织21.7%(10/46),P<0.05].低分化转移灶HO-1表达水平显著高于中、高分化转移灶(P<0.05).Western印迹法检测胃癌腹膜转移患者的转移灶HO-1表达水平显著高于其癌旁无瘤腹膜组织(P<0.05).HO-1在耐药细胞系GC9811-P的表达水平较其亲本细胞系明显上调(P<0.05).结论 HO-1在胃腺癌腹膜转移过程中表达增高,HO-1可能参与胃癌腹膜转移发生.高表达HO-1与胃癌腹膜转移组织的恶性程度有关,其信号转导通路可能存在于组织的上皮细胞,并可能与多药耐药有关.

Expression of heme oxygenase-1 in peritoneal metastasis of gastric cancer and its significance HUI

Objective To investigate the expression and significance of heme oxygenase-1(HO-1) in gastric adenocarcinoma and its peritoneal metastatic tissues, as well as drug-resistant cell strains. Methods The expression of HO-1 in patients with (n=68) or without (n=46) peritoneal metastasis of gastric adenocarinoma was examined. The expression of HO-1 in cancerous tissue, peritoneal metastatic foci, and normal peritoneum was detected by immunohistochemistry. The expression of HO-1 protein in metastatic foci and drug-resistant cell strains was measured by Western blotting. Results The positive expression of HO-1 was 39.7% (27/68) in gastric adenocarcinoma tissues with metastasis and 41.2% (28/68) in peritoneal metastatic tissues, which was significantly higher than that in normal peritoneum (0%,0/68,P<0.01) and gastric adenocarcinoma tissues without metastasis (21.7%, 10/46, P<0.05). The Western blot study showed that the HO-1 expression in metastatic tissues was higher than that in normal peritoneum (P<0.05). The expression of HO-1 protein was markedly increased in GC9811-P drug-resistant cell strains compared with its parental cell strains (P<0.05). Conclusions The increased expression of HO-1 may be involved in the peritoneal metastasis of gastric adenocarcinoma, and related to the malignant potential. The underlying signal pathways in neopastic epithelium may also be related to the multi-drug resistance.