| 铜绿假单胞菌密度感应系统自体诱导因子3OC12-HSL对人肠道上皮细胞Caco-2凋亡的影响及其机制 |
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| 引用本文： | 叶露,李洪涛,王丽丽,邢铭友,宋建新. 铜绿假单胞菌密度感应系统自体诱导因子3OC12-HSL对人肠道上皮细胞Caco-2凋亡的影响及其机制[J]. 中华微生物学和免疫学杂志, 2009, 30(10): 506-510. DOI: 10.3760/cma.j.issn.0254-5101.2010.06.003 |
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| 作者姓名： | 叶露 李洪涛 王丽丽 邢铭友 宋建新 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院感染科,武汉,430030; |
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| 摘 要： | Objective To investigate the effect of quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa on the apoptosis of Caco-2 cells and its mechanism. Methods Caco-2 cells were incubated with 3OC12-HSL for 4 h and then examined by MTT method for cytoactivities. Flow cytometry was used to analyze apoptosis rate of Caco-2 cells. Expression of apoptosis associated proteins p-p38/MAPK and NF-κB were detected by Western blot. Results After exposure to 3OC12-HSL for 4 h, cytoactivities of Caco-2 cells was reduced(P<0.05) with dose-dependent pattern, and higher dose of 3OC12-HSL leaded to increasing apoptosis rate of Caco-2 cells(P<0.05). 3OC12-HSL raised expression of apoptosis associated proteins p-p38/MAPK and NF-κB detected by Western blot. Conclusion Quorum sensing autoinducer 3OC12-HSL can effect cytoactivities of Caco-2 cells and may induce its apoptosis by enhancing the expression of p-p38/MAPK and NF-κB protein.
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| 关 键 词： | 密度感应系统 细胞凋亡 Caco-2细胞 |
Quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa induces the apoptosis of Caco-2 cells |
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| YE Lu,LI Hong-tao,WANG Li-li,XING Ming-you,SONG Jian-xin. Quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa induces the apoptosis of Caco-2 cells[J]. Chinese Journal of Microbiology and Immunology, 2009, 30(10): 506-510. DOI: 10.3760/cma.j.issn.0254-5101.2010.06.003 |
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| Authors: | YE Lu LI Hong-tao WANG Li-li XING Ming-you SONG Jian-xin |
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| Abstract: | |
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| Keywords: | 3OC12-HSLp38/MAPKQuorum sensing system3OC12-HSLApoptosis p38/MAPKCaco-2 cells |
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