70例手足口病病原检测和临床分析 |
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引用本文: | 张欣,闫惠平,黄春,檀玉芬,马冬梅,张海萍,刘妍,王曙照. 70例手足口病病原检测和临床分析[J]. 中华预防医学杂志, 2009, 43(8): 872-874. DOI: 10.3760/cma.J.Issn.0253-9624.2009.10.008 |
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作者姓名: | 张欣 闫惠平 黄春 檀玉芬 马冬梅 张海萍 刘妍 王曙照 |
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作者单位: | 首都医科大学附属北京佑安医院感染与免疫研究中心,100069; |
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摘 要: | Objective To analyze the etiology and clinical manifestations of 70 patients with hand-foot-mouth disease (HFMD). Methods The viral RNA from the pharynx swab samples were extracted and collected from 70 HFMD patients (of which,60 cases were under 5 years old) to detect the 5'-UTR gene of EV,the Vp3-Vp1 genes of EV71 and Cox-A16 by utilizing the technique of RT-PCR. Results Of 70 HFMD patients,30 cases (42.8%, 30/70) were positive in enterovirus nucleic acid, including EV71 and Cox-A16,pharynx swab sample testing. While, of the enteroviral pathogen RNA-positive 30 cases, EV71 accounted for 66. 7% (20/30). The proportion of etiological positive of 39 samples collected within 4 days after onset was 66.7% (26/39), but the probability of 31 samples collected after more than 5 days after onset was 12.9%(4/31),the difference was significantly(χ2=20.4,P<0. 01). Conclusion HFMD patients were mainly infants and young children between 0-5 years old, but adults could also be attacked. The enterovirus detected was mainly EV71,and the pharynx swab samples should be collected within 4 days after onset to increase the related viral nucleic acid positive detection probability.
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关 键 词: | 手足口病 肠道病毒感染 逆转录聚合酶链反应 评价研究 |
The etiology and clinical manifestations of 70 patients with hand-foot-mouth disease |
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