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二甲基三十六烷基铵及卡介苗多糖核酸佐剂在结核亚单位疫苗加强BCG免疫中的效应研究
引用本文:达泽蛟,胡丽娜,王秉翔,姜雯雯,傅林锋,于红娟,雒彧,祝秉东. 二甲基三十六烷基铵及卡介苗多糖核酸佐剂在结核亚单位疫苗加强BCG免疫中的效应研究[J]. 中华微生物学和免疫学杂志, 2009, 30(10): 555-559. DOI: 10.3760/cma.j.issn.0254-5101.2010.06.016
作者姓名:达泽蛟  胡丽娜  王秉翔  姜雯雯  傅林锋  于红娟  雒彧  祝秉东
作者单位:兰州大学结核病研究中心暨病原生物学研究所,730000;兰州生物制品研究所;
基金项目:重大传染病防治科技重大专项国家高技术研究发展计划(863计划)
摘    要:Objective To investigate the adjuvant effect of dimo-thylidioctyl ammonium bromide (DDA) and/or DDA-BCG polysaccharide nucleic acid( BCG-PSN), which was combined with a Mycobacterium tuberculosis fusion protein AMM ( Ag 8 5 B - MPT64190-198 - Mtb8.4 ) to boost BCG primed immunization. Methods DDA with or without BCG PSN was mixed with the fusion protein AMM to construct the boosting vaccine. Mice were immunized with BCG and then boosted twice with AMM formulated with the adjuvant DDA with or without BCG-PSN. PBS or BCG vaccination without boosting was used as control. The humoral and cell-mediated immune responses were analyzed by ELISA and ELISPOT. Moreover, the protective efficacy of BCG prime-AMM subunit vaccine boosting against Mycobacterium tuberculosis infection was analyzed. Results With in vitro stimulation of Ag85B and PPD( purified protein derivative) antigen, the number of IFN-γ secreting cells from the mice boosted twice by AMM/DDA/BCG-PSN and AMM/DDA were higher than BCG and PBS group (P <0.05). The CFU in lungs of mice boosted with AMM/DDA/BCG-PSN was less than that of PBS group(P <0.05), while the CFU of AMM/DDA-boosted mice was less than that of BCG and PBS group(P < 0.05).However, fewer lesions were seen in lungs of mice immunized with BCG alone or BCG-prime-AMM/DDA/BCG-PSN boosting than the other groups. Conclusion DDA is an idea adjuvant for tuberculosis subunit vaccine;BCG-PSN might play a role in alleviating the immunity-mediated pathology.

关 键 词:结核分枝杆菌   亚单位疫苗   佐剂   

DDA and BCG polysaccharide nucleic acid improved the immunogenicity and protective efficacy of tuberculosis subunit vaccine against Mycobacterium tuberculosis infection in mice
DA Ze-jiao,HU Li-na,WANG Bing-xiang,JIANG Wen-wen,FU Lin-feng,YU Hong-juan,LUO Yu,ZHU Bing-dong. DDA and BCG polysaccharide nucleic acid improved the immunogenicity and protective efficacy of tuberculosis subunit vaccine against Mycobacterium tuberculosis infection in mice[J]. Chinese Journal of Microbiology and Immunology, 2009, 30(10): 555-559. DOI: 10.3760/cma.j.issn.0254-5101.2010.06.016
Authors:DA Ze-jiao  HU Li-na  WANG Bing-xiang  JIANG Wen-wen  FU Lin-feng  YU Hong-juan  LUO Yu  ZHU Bing-dong
Abstract:
Keywords:Mycobacterium tuberculosisSubunit vaccineAdjuvant
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