PTTG mRNA及其蛋白在食管鳞癌中的表达及临床意义

目的:探讨食管鳞癌组织中PTTG mRNA与蛋白的表达及其二者间的相互关系。研究它们的表达与肿瘤临床资料之间的联系。方法:采用原位杂交技术检测30例食管鳞癌及相应的癌旁组织中PTTGmRNA的表达,同时采用免疫组织化学SP法检测相应标本中PTTG蛋白的表达。结果:在食管鳞癌和相应的癌旁组织中PTTG mRNA表达的阳性率分别为66.7%(20/30),10%(3/30)。PTTG蛋白表达的阳性率分别为76.7%(23/30),16%(5/30)。食管鳞癌组远高于癌旁组,有显著性差异(P<0.01)。PTTG mRNA与蛋白在食管鳞癌组织的阳性表达具有一致性(P>0.05),其阳性率高低与性别、年龄、肿瘤大小无关,而与组织学分级、淋巴结转移、TNM分期有关。结论:PTTG基因的异常表达可能对食管鳞癌的发生和发展起重要作用,它可能为食管鳞癌的早期诊断和基因治疗提供新的靶点。

The Expression of Pituitary Tumor Transforming Gene mRNA and Its Protein In Esophageal Squamous Carcinoma and Its significance

Objective:To investigate the expression of pituitary tumor transforming(PTTG) mRNA and protein in esophageal squamous carcinoma(ESC) and its correlationship with clinical data.Methods: In 30 cases of ESC,the expression of PTTG mRNA was detected by in situ hybridization(ISH) and the expression of PTTG protein by immunohistochemical method and SP technique in cancerous tissues and paraneoplastic tissues.Results: In cancerous tissues and paraneoplastic tissues of ESC,the positive expression of PTTG mRNA was respectively 66.7% and 10%,the positive expression of PTTG protein was respectively 76.7% and 16%,showing significant difference(P<0.01).The positive expression of PTTG mRNA in ESC tissues was similar to that of PTTG protein(P>0.05),which was not related to sex,age and the size of tumor,but related to histological grade,lymphatic metastasis and TNM staging.Conclusion: The abnormal expression of PTTG gene may play important role in the genesis and development of ESC.It will be a new target in the early diagnosis and gene therapy for ESC.