| TGF-β1诱导的肝癌细胞SMMC-7721上皮间质转化对细胞Wnt/β-catenin表达的影响 |
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| 引用本文： | 王勇,范飞,刘斌. TGF-β1诱导的肝癌细胞SMMC-7721上皮间质转化对细胞Wnt/β-catenin表达的影响[J]. 肿瘤药学, 2013, 0(5): 348-352 |
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| 作者姓名： | 王勇 范飞 刘斌 |
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| 作者单位： | [1]徐州医学院研究生院,江苏徐州221042 [2]连云港市东方医院普外科,江苏连云港,222042 [3]徐州医学院附属医院肿瘤外科,江苏徐州221042 [4]第二军医大学东方肝胆外科医院胆道二科,上海200438 |
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| 摘 要： | 目的探讨TGF-β1诱导的肝癌细胞SMMC-7721上皮间质转化对细胞信号通路蛋白Wnt/β-catenin表达的影响。方法采用5μg·mL-1 TGF-β1诱导SMMC-7721发生上皮间质转化,分别采用RT-PCR和Western blot法检测细胞中Wnt1、β-catenin、Vimentin mRNA和总蛋白的表达。结果 TGF-β1能显著诱导肝癌细胞SMMC-7721发生上皮间质转化,细胞中Wnt1、β-catenin、Vimentin mRNA和相应总蛋白的表达均显著增加(P<0.05)。结论 TGF-β1诱导肝癌细胞SMMC-7721上皮间质转化时可促进细胞Wnt/β-catenin的表达。
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| 关 键 词： | 肝癌 上皮间质转化 转化生长因子β1 Wnt/β-catenin信号通路 |
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| -------------. ------------------------[J]. Anti-Tumor Pharmacy, 2013, 0(5): 348-352 |
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| Authors: | ------------- |
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| Affiliation: | . (lXuzhou Medical College, Xuzhou, Jiangsu, 221042, China; 2The Oriental Hospital of Lianyungang, Lianyungang, Jiangsu, 222042, China; 3Department of Biliary Surgery II. Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200438, China; 4The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, 221042, China) |
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| Abstract: | Objective To investigate the influence of TGF-β1-induced epithelial mesenchymal transition （EMT） on the ex-pression of Wnt/β-catenin in signaling pathway in human hepatocellular carcinoma cell line SMMC-7721. Methods EMT was induced by 5μg·mL-1 of TGF-β1 in SMMC-7721, and the mRNA and protein expressions of Wnt1,β-catenin and Vimen-tin were detected by RT-PCR and Western blot. Results TGF-β1 can strongly induce the EMT in hepatocellular carcinoma SMMC-7721, and the mRNA and protein expressions of Wnt1,β-catenin and Vimentin all remarkably increased （P〈0.05）. Conclusion EMT induced by TGF-β1 could promote the expression of Wnt/β-catenin in human hepatocellular carcinoma cell line SMMC-7721. |
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| Keywords: | Hepatocenular carcinoma Epithelial mesenchymal transition Transforming growth factor-β （TGF-β1） Wnt/β-catenin signaling pathway |
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