53例慢性化脓性中耳炎患者分泌物细菌培养及药敏学分析

目的 分析53例慢性化脓性中耳炎患者分泌物细菌分布及药敏学的数据结果, 探讨此类患者的临床用药方案。方法 采用VITEK-2 COMPACT微生物分析系统对近3年住院的53例慢性化脓性中耳炎患者分泌物进行病原菌分离鉴定与药敏试验, 进行总结统计。结果 53份分泌物培养出微生物的有45份, 检出率为84.9%, 共培养出微生物49株, 其中革兰阳性球菌21株, 占42.9%;革兰阴性杆菌 21株, 占42.9%;真菌7株, 占14.2%。检出主要病原菌为铜绿假单胞菌, 占22.4%, 其次为表皮葡萄球菌、金黄色葡萄球菌, 分别占16.3%、14.3%。常见革兰阳性菌、革兰阴性菌对临床常用药物左氧氟沙星均有较高敏感性(分别为81.0%、90.9%)。结论 铜绿假单胞菌、表皮葡萄球菌、金黄色葡萄球菌是常见慢性化脓性中耳炎感染细菌, 对临床常用药物(如左氧氟沙星)有较高敏感性。而对常用药物治疗不敏感的病例要警惕细菌耐药可能, 及时行细菌培养, 真菌感染不容忽视。

Pathogenic bacteria and drug sensitivity in 53 patients with chronic suppurative otitis media

Objective To investigate the pathogenic bacteria and drug sensitivity tests in patients with chronic suppurative otitis media for drug application. Methods Secretion were collected from 53 patients with chronic suppurtive otitis media who were admitted into our hospital from March 2011 to March 2014.Bacterial culture and drug susceptibility tests using VITEK-2 COMPACT microbiological analysis system were performed. Results There were 49 stains of microbes isolated from 45 secretion specimens of middle ear. The first three most common pathogenic bacteria were Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus aureus accounted for 22.4%, 16.3%, 16.3%, respectively. The common gram-positive and gram-negative bacteria all showed highly sensitive to levofloxacin. (81%, 90.9% correspondingly). Conclusion Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus aureus are main pathogenic bacteria for chronic suppurative otitis media in our region, which are highly sensitive to general antibiotics. Culture and drug sensitivity test have great value for clinical drug application. The fungus infection can't be neglected.