大肠腺癌p16抑癌基因发迹的免疫组化研究

研究大肠腺癌中抑癌基因ｐ１６的表达情况。方法：用免疫组化ＬＳＡＢ法检测７１例结肠癌和直肠癌存档标本中ｐ１６蛋白的表达。结果：３６例（５０．７０％）标本呈ｐ１６阳性，阳性细胞往往比阴性细胞有更明显的异型性，ｐ１６阳性与临床病理分级有关。结论大肠癌的ｐ１６基因改变表现出异质性，即一部分肿瘤细胞为ｐ１６失活或表达不增高，另一部分则为ｐ１６过度表达。ｐ１６阳性肿瘤细胞在形态上的异型性特征以及ｐ１６阳性标本

Immunohistochemical Study of p16 Gene Alteration in Colorectal Cancer

Background/Aims: To study the alteration of p16 gene in colorectal cancer. Methods: Immunohistochemistry with LSAB method was used in 71 archived colon and rectum cancer specimens.Results: 36 cases (50.70%) of specimens were positive for p16. The p16-positive adenocarcinoma cells displayed more heteromorphosis than those negative ones, a relationship between p16 positivity and the tumor grading was found. Conclusions: Alteration of p16 gene in the colorectal carcinoma is heterogenous, in which p16 may be inactivated or not overexpressed in some tumor cells while overexpressed in others. The morphological characteristic of p16-positive cells and higher frequency of p16positivity in the specimens with low degree differentiation indicate an association of p16 overexpression with abnormal proliferation and differentiation of colorectal cancer.