恶性腹水检测DNA异倍体的临床意义

目的评价应用DNA异倍体指标诊断恶性腹水的价值。方法良、恶性腹水各57份,应用流式细胞术(FCM)检测DNA异倍体、磁性抗体分离酶联免疫测定技术(MAIA)检测CEA,比较它们诊断恶性腹水价值的差别。结果良性与恶性腹水组组间DNA异倍体、CEA阳性结果(42与3、33与6)的差别均具有极显著性(P<0.001),恶性组内DNA异倍体的阳性率高于CEA。DNA异倍体与CEA指标诊断恶性腹水的特异性、敏感性、阳性预测值、阴性预测值、准确性分别为:94.7%与89.5%;73.7%与57.9%;93.3%与84.6%;75%与63%;84.2%与73.7%。结论检测腹水中DNA异倍体及CEA指标诊断恶性腹水具有较高的价值,其中DNA异倍体指标的诊断价值高于CEA。

The Clinical Significance on the Detection of DNA Aneuploid in Malignant Ascites

Objective To evaluate the value of DNA aneuploid in diagnosis of malignant ascites.Methods Flow cytometry and magnetic antibody immunoenzymetric assay were used to detect DNA aneuploid and CEA in 57 cases of malignant ascites (malignant group) and 57 cases of benign ascites (benign group) and to compare the difference of diagnostic value between the two index.Results The positive results of DNA aneuploid and CEA were 42 and 3, 33 and 6 in malignant and benign group and the difference of the positive results between the two groups had statistical meaning (P<0.001). The specificities, sensitivities, positive predictive values, negative predictive values and accuracies of the two indexes were DNA aneuploid: 94.7%, 73.7%, 93.3%, 75% and 84.2%; CEA: 89.5%, 57.9%, 84.6%, 63% and 73.7%.Conclusion DNA aneuploid and CEA are useful index for the diagnosis of malignant ascites and the value of DNA aneuploid is higher than CEA.