Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae |
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Authors: | Bun-ichiro Ono Chinatsu Heike Yukie Yano Toyomi Inoue Kazuhide Naito Satoru Nakagami Akio Yamane |
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Institution: | (1) Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, 700 Okayama, Japan;(2) Institute for Biotechnology Research, Wakunaga Pharmaceutical Co. Ltd., Koda-cho, Takata-gun, 729-64 Hiroshima, Japan |
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Abstract: | Summary A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 m DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII. |
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Keywords: | Saccharomyces cerevisiae Cysteine biosynthetic CYS4 Mapping |
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