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| 膝关节骨性关节炎患者外周血自然杀伤细胞活性测定的临床分析 | |
| 引用本文： | 梁广玉,王爱国,于占芝,邓爱民,李学峰,李志怀,裴银辉.膝关节骨性关节炎患者外周血自然杀伤细胞活性测定的临床分析[J].河北医药,2012,34(23):3527-3529. |
| 作者姓名： | 梁广玉 王爱国 于占芝 邓爱民 李学峰 李志怀 裴银辉 |
| 作者单位： | 1. 河北省迁安市人民医院骨科,064400 2. 河北省唐山市路北区社区卫生服务中心 3. 承德医学院附属医院骨科 4. 河北联合大学医学院生命科学系 |
| 基金项目： | 唐山市科学技术研究与发展指导计划项目（编号：10130280c） |
| 摘 要： | 目的研究自然杀伤(NK)细胞活性变化与膝关节骨性关节炎(knee osteoarthrosis,K-OA)的关系。方法选择K-OA患者50例,作为K-OA组,无关节炎病史及自身免疫病者41例作为对照组,补体裂解法分离K-OA组与对照组外周血NK细胞,酶免疫标记技术测定两种不同NK细胞亚群比例,MTT法检测NK细胞杀伤活性。结果与对照组比较,K-OA组CD16-CD5+6比例减少,而CD1+6CD5+6比例上升(P<0.05),NK细胞杀伤活性增强。结论 K-OA的发生与具有杀伤活性的CD1+6CD5+6NK细胞亚群比例上升,杀伤活性增强有关。 |
| 关 键 词： | 膝关节骨性关节炎 外周血 自然杀伤细胞 亚群 杀伤活性 |
Study on the activity of peripheral blood NK cell in patients with knee osteoarthrosis |
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| Institution: | LIANG Guangyu, WANG Aiguo , YU Zhanzhi,et al. People＇ s Hospital of Qian＇ an City ,Hebei, Qian ＇ an 064400, China |
| Abstract: | Objective To investigate the correlation between the activity of nature killer （NK） cell in peripheral blood and knee osteoarthrosis （ K-OA ）. Methods 50 patients with K-OA were enrolled in K-OA group,and 41 patients without arthritis history and autoimmune diseases were enrolled in control group. The NK cells were separated by means of complement lysis method, then the proportion of two kinds of NK cell was detected, and the killing activity of NK cell was detected by MTT. Results As compared with control group, the proportion of CD16- CD56＋ was decreased,however,the proportion of CD16＋ CD56＋ was increased （ P 〈 0.05 ） ,and the activity of NK cell was enhanced. Conclusion The pathogenesis of K-OA is correlated with the increase of the proportion of CD16 ＋ CD56 ＋ NK cell subgroup and with the increase of the killing activity of NK cells. |
| Keywords: | knee osteoarthrosis peripheral blood nature killer cells subgroup killing activity |
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