|
|||||
|
|
| TCR Vβ8/pcDNA3.1重组质粒的构建 | |
| 引用本文： | 张明智,赵国强,李继昌,董子明,李学民. TCR Vβ8/pcDNA3.1重组质粒的构建[J]. 郑州大学学报(医学版), 2002, 37(3): 268-273 |
| 作者姓名： | 张明智 赵国强 李继昌 董子明 李学民 |
| 作者单位： | 1. 郑州大学第一附属医院肿瘤科,郑州,450052 2. 郑州大学基础医学院免疫学教研室,郑州,450052 3. 郑州大学第一附属医院消化内科,郑州,450052 4. 郑州大学基础医学院病理生理学教研室,郑州,450052 5. 郑州大学第一附属医院检验科,郑州,450052 |
| 摘 要： | 目的：构建重组质粒TCRVβ8／pcDNA3．1。方法：从人淋巴瘤Jurkat细胞中提取mRNA，经RT－PCR法获取TCRVβ基因；将表达质粒pcDNA3．1和TCRβ基因行BamHI和HindⅢ双酶切、低熔点胶纯化、连接酶切产物、转化DH5a感受态细菌、筛选菌落和测序鉴定。结果：电泳获得523bp的TCRVβ8预期条带，测序证实为正确的TCRVβ目的基因序列。结论：测是构建TCRVβ8／pcDNA3．1重组质粒的重要步骤。 |
| 关 键 词： | 淋巴瘤 TCR 独特型 重组质粒 DNA疫苗 |
| 修稿时间： | 2002-01-30 |
Construction of recombinant plasmid of TCR β8/pcDNA3.1 |
|
| ZHANG Mingzhi,ZHAO Guoqiang,LI Jichang,DONG Ziming,LI XueminDepartment Ontology of,the First Affiliated Hospital,Zhengzhou University,Zhengzhou Immunology Department,Basic Medical College,Zhengzhou University,Zhengzhou. Construction of recombinant plasmid of TCR β8/pcDNA3.1[J]. Journal of Zhengzhou University: Med Sci, 2002, 37(3): 268-273 | |
| Authors: | ZHANG Mingzhi ZHAO Guoqiang LI Jichang DONG Ziming LI XueminDepartment Ontology of the First Affiliated Hospital Zhengzhou University Zhengzhou Immunology Department Basic Medical College Zhengzhou University Zhengzhou |
| Affiliation: | ZHANG Mingzhi,ZHAO Guoqiang,LI Jichang,DONG Ziming,LI XueminDepartment Ontology of,the First Affiliated Hospital,Zhengzhou University,Zhengzhou450052 Immunology Department,Basic Medical College,Zhengzhou University,Zhengzhou450052 Department of |
| Abstract: | |
| Keywords: | lymphoma TCR idiotype recombinant plasmid DNA vaccine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |