贴壁型树突细胞的分离培养及鉴定

目的探索树突细胞的生长状态,建立鉴别树突细胞的简便方法。方法从正常人外周血分离获得单个核细胞,常规培养2 h,取贴壁细胞,用含细胞因子的培养液进行培养,于第1、2、5、7天在光镜下对其进行形态鉴别,然后检测其促淋巴细胞增殖和诱导免疫反应的功能。结果实验中所培养的树突细胞始终处于贴壁状态,未见悬浮生长现象,其形态与理论相符,可在体外促进淋巴细胞增殖,并诱导免疫反应。结论树突细胞体外培养至发育成熟的过程中,树突细胞可能始终处于贴壁生长状态。

Fractional Cultivation and Identification of Attached Dendritic Cells

Objective To explore the possible growth state of DCs and establish a convenient approach that detects DCs.Methods We separated mononuclearcell from health adult＇s peripheral blood,and cultured fo r 2 hours conventionally.The attached cell was cultured with culture solution co ntaining cytokine.Cells＇ appearance was observed by light microscope on the fir st,twice,fifth and seventh day.Then we detected the cells＇ function to promote achroacyte multiplication and induce immunoreactive.Results The DCs we cultured kept attached state all the time without suspension.Their appea rance matched the theory.This kind of cells could promote achroacyte multiplicat ion and induce immunoreactive successfully.Conclusion In the pr ocess of fractional cultivation,DCs may keep attached state all the while.