| 江西巴蜗牛活性成分提取方法的比较研究 |
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| 引用本文： | 刘小燕,李朝品.江西巴蜗牛活性成分提取方法的比较研究[J].热带病与寄生虫学,2005,3(3):151-152. |
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| 作者姓名： | 刘小燕 李朝品 |
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| 作者单位： | 232001,安徽淮南市,安徽理工大学医学院;232001,安徽淮南市,安徽理工大学医学院 |
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| 摘 要： | 目的比较江西巴蜗牛生物活性成分不同提取方法间的差异,以求找到一种简单、可行和有效的提取方法。方法分别利用碱提法、酶提法及碱加酶法提取,并用Sevage法脱蛋白,苯酚—硫酸法比色测定天螺多糖总糖含量。结果三种方法均得到白色颗粒状结晶物质,其中碱加酶提法的得率(0.32%)和总糖含量(80.8%)最高。结论碱加酶法是一种可行、方便和有效的提取方法。
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| 关 键 词： | 江西巴蜗牛 天螺多糖 提取方法 |
| 修稿时间： | 2005年7月11日 |
Research on the extraction methods of active components from Bradybaena of Jiangxinensis |
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| Liu xiaoyan,li chaopin.Research on the extraction methods of active components from Bradybaena of Jiangxinensis[J].Journal of Tropical Diseases and Parasitology,2005,3(3):151-152. |
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| Authors: | Liu xiaoyan li chaopin |
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| Institution: | Liu xiaoyan,li chaopin. School of medicine,Anhui University of Science and Technology,Huainan Anhui,232001,China. |
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| Abstract: | Objective To compare the differences between different extraction methods of active components from Bradybaena of Jiangxinensis, in order to find a simple and efficient method. Methods Three methods of base-extraction, enzyme -extraction and base-enzyme extraction were applied to extract Tianluo Polysaccharide, and then remove protein by method of Sevage, finally the spectrophotometric method of phenol-sulfuric acid was used to detected total sugar content. Results Extractions from Bradybaena of Jiangxinensis by three methods were all White granulo-crystal matters. Tianluo Polysaccharide extracted by base-enzyme extraction has the highest yield and total sugar content (0.32% ,80.8%). Conclusion Base-enzyme extraction was a simple, rapid and well reproducible method. |
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| Keywords: | Bradybaena of Jiangxinensis Tianluo Polysaccharide Extraction |
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