Hp感染与胃炎性病变,不典型增生及胃癌关系的研究

目的　通过检测 392例不同胃部疾病 :慢性胃炎 (2 13例 )、消化性溃疡 (77例 )、不典型增生 (5 3例 )及胃癌(4 9例 )中幽门螺杆菌 (Hp)感染的不同阳性率 ,探讨幽门螺杆菌感染与胃部疾病的关系。方法　应用快速尿素酶法 ,Giemsa染色法及C1 4呼气实验等 3种不同方法检测胃粘膜Hp感染。应用免疫组织化学ABC法检测人胃癌组织中caspase 3和bcl xl的表达情况。并对比不同胃粘膜病变中Hp阳性率及caspase 3和bcl xl表达的差异。结果　Hp感染与胃炎性病变及不典型增生的严重程度密切相关 ,但在胃癌中的检出率较前二者低 (P <0 0 5 )。胃炎性病变、不典型增生及胃癌中caspase 3的表达依次降低 (P <0 0 5 ) ,bcl xl的表达依次升高 (P <0 0 5 )。Hp阳性组较Hp阴性组caspase 3表达降低 (P <0 0 5 ) ,而bcl xl表达升高 (P <0 0 5 )。结论　Hp感染与胃粘膜炎性病变及不典型增生程度正相关 ,Hp在胃癌的发生发展过程中可能是早期事件 ,可能与改变细胞凋亡有关

Relationship between Hp infection and inflammation,dysplasia and carcinoma of stomach

Objective Hp infection in 392 cases of stomach lesions(213 cases of gastric inflammation, 76 cases of dysplasia and49 cases of gastric carcinoma) was detected to explore the potential relationship between Hp infection and stomach lesions. Methods Rapid urease method, Giemsa staining and C 14 breathing test were applied to detect the Hp infection in gastric mucosa. Hp infection rates were compared between different stomach lesions. Results There is a close relationship between Hp infection rate and severity of gastric mucosa dysplasia. But Hp infection rate was lower in gastric cancer than in other stomach lesions. Caspase 3 positively correlates with the severity of gastric lesion, while bcl xl negatively does. Conclusion Hp infection rate paralleled to severity of gastric mucosa lesion and dysplasia. Hpinfection maybe is a forepart affair, which interferes with mucosa apoptosis during carcinogenesis and progress of gastric cancer.