压力作用下大鼠颞下颌关节滑膜细胞超微结构的变化

目的 探讨在应力作用下颞下颌关节(temporomandibular joint,TMJ)滑膜细胞超微结构的变化及意义.方法 大鼠TMJ滑膜细胞原代培养,透射电镜下观察不同静水压力(0、30、60、90 kPa)作用12 h后细胞超微结构的改变.结果 大鼠30 kPa压力下细胞超微结构变化不明显;60 kPa压力下细胞胞质紧密围绕胞核,呈新月形,线粒体轻度肿胀;90 kPa压力下,核内结构溶解,胞质内空泡形成,有类似包涵体的"凋亡小体"存在,部分核膜结构不完整.结论 滑膜细胞在压力作用下超微结构会发生凋亡样变化,且这种变化程度与压力大小有关.

Effect of hydrostatic pressure on ultrastructure of synovial fibroblasts from rat temporomandibular joint

Objective To investigate the role of apoptosis in the remodeling of temporomandibular ioint(TMJ)under pressure.nethods Synovial fibroblaslt obtained from rat temporomandibular joint were subiected to different hydrostatic pressure for 12 h. Changes of uhrastmeture were observed by transmission electron microscope.Results At 30 kPa,the uhlastructure of synovial fibroblasit showed no obvious changes.At 60 kPa.the chromatin was condensated,the karyon took on crescent and the mitochondria seemed varicose.At 90 kPa,the apoptosis-like body was wrapped by membrane and embedded in the high density chromatin.Condusions Apoptosis-like change took place in ultrastructure of synodal fibroblasts under hydrostatic pressure,and the degree of the change was related to the hydrostatic pressure exerted.