| 苦参碱体外诱导人肝癌细胞系Bel-7404分化及机制 |
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| 引用本文： | 王涌,吴育连,刘颖斌,唐喆.苦参碱体外诱导人肝癌细胞系Bel-7404分化及机制[J].中华中医药杂志,2009(7). |
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| 作者姓名： | 王涌 吴育连 刘颖斌 唐喆 |
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| 作者单位： | 浙江大学医学院附属二院; |
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| 基金项目： | 浙江省医药卫生科研基金项目(No.2003B07) |
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| 摘 要： | 目的:研究苦参碱对人肝癌细胞系体外诱导分化作用,并探讨其作用机制。方法:不同浓度苦参碱体外作用于Bel-7404细胞,噻唑蓝比色法(MTT法)绘制生长曲线,光镜下观察细胞形态变化,分别以软琼脂集落形成试验、细胞黏附试验、运动试验、侵袭试验检测细胞生物学特性变化,以流式细胞仪分析细胞周期变化。结果:0.5-1.0g/L苦参碱作用于Bel-7404细胞后,明显抑制细胞增殖,细胞形态向正常转化,克隆形成率显著降低,细胞黏附力明显上升,运动力和侵袭力则显著下降。细胞周期分析G1期细胞堆积,G2和S期细胞显著降低。结论:一定浓度的苦参碱具有抑制Bel-7404细胞生长增殖和诱导分化作用,其作用机制可能与细胞周期调节有关。
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| 关 键 词： | 苦参碱 肝细胞癌 诱导分化 |
Experimental study on differentiation of human hepatocellular carcinoma cell line Bel-7404 induced by matrine in vitro and its mechanism |
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| WANG Yong,WU Yu-lian,LIU Ying-bin,TANG Zhe.Experimental study on differentiation of human hepatocellular carcinoma cell line Bel-7404 induced by matrine in vitro and its mechanism[J].China Journal of Traditional Chinese Medicine and Pharmacy,2009(7). |
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| Authors: | WANG Yong WU Yu-lian LIU Ying-bin TANG Zhe |
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| Institution: | WANG Yong,WU Yu-lian,LIU Ying-bin,TANG Zhe(Department of General Surgery,Second Affiliated Hospital of Zhejiang University,Hangzhou 310009,China) |
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| Abstract: | Objectives:To investigate the differentiation of human hepatocellular carcinoma cell line Bel-7404 induced by Matrine in vitro,and discuss its mechanism.Methods:After dealing human hepatocellular carcinoma cell line Bel-7404 with different concentrations of Matrine,we drew cell growth curve by MTT colorimetry,and cell morphology was examined by light microscope.Then we evaluated the tumor cell biological characteristics changes through improved soft agarose clone forming test,cell adhesion assay,cell moveme... |
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| Keywords: | Matrine Hepatocellular carcinoma Induced differentiation |
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