蟾酥脂质体注射液的抗肿瘤作用

目的探讨蟾酥脂质体注射液(CS)的抗肿瘤作用。方法体内实验采用小鼠肝癌H22、肉瘤S180荷瘤小鼠模型,考察CS的抑瘤作用;采用小鼠接种肝癌H22后,用环磷酰胺造成免疫低下模型,考察CS提高机体免疫力作用。体外实验采用MTT法,测CS对人宫颈癌HeLa细胞或人肝癌BEL-7402细胞增殖抑制率。结果CS(0.2、0.4、0.8 mg.kg-1)对2种荷瘤小鼠肿瘤增长有显著或非常显著的抑制作用(P<0.05,P<0.01);同时对机体具有明显的免疫调节作用。CS 0.01~3.00 mg.L-1对人宫颈癌HeLa细胞或肝癌BEL-7402细胞增殖有非常显著的抑制作用;CS对人宫颈癌HeLa细胞IC50为0.51 mg.L-1,95%可信限0.213 2~1.214 0 mg.L-1,CS对人肝癌BEL-7402细胞IC50为0.75 mg.L-1,95%可信限0.312 6~1.801 5 mg.L-1。结论蟾酥脂质体注射液体内、体外均有抗肿瘤活性,且具有免疫调节功能。

Antitumor effect of Venenum Bufonis liposome injection

Objective To observe antitumor effect of Venenum Bufonis liposome injection(CS).Methods In vivo,the models of hepatocarcinoma 22(H22),sarcoma 180(S180) in mice and immune function inhibited mice induced by Cytoxan were establisehd.In vitro,time and dose effects of CS in HeLa cell and BEL-7402 human hepatocarcinoma cell lines were detected using MTT assay.Results The studies demonstrated that CS(0.2,0.4,0.8 mg·kg-1) had obvious antitumor effects on H22 and S180(P<0.05,P<0.01),and had activities of immunoregulation;CS(0.01-3.00 mg·L-1)could inhibit proliferation of HeLa cells(IC50:0.51 mg·L-1,95%confidence interval:0.213 2-1.214 0 mg·L-1) and BEL-7402 cells(IC50:0.75 mg·L-1,95%confidence interval :0.312 6-1.801 5 mg·L-1).Conclusions CS has antitumor effects both in vivo and in vitro.It also shows immunoregulation activities.