| 差示聚合酶链式反应检测骨髓转移的神经母细胞瘤N－myc扩增及临床意义 |
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| 引用本文： | 杨朝晖,张锦华,杨学忠,陈世浩,张学,金春莲,姜莉,孙开来.差示聚合酶链式反应检测骨髓转移的神经母细胞瘤N－myc扩增及临床意义[J].中华小儿外科杂志,1996(4). |
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| 作者姓名： | 杨朝晖 张锦华 杨学忠 陈世浩 张学 金春莲 姜莉 孙开来 |
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| 作者单位： | 中国医科大学第二临床学院血液研究室，中国医科大学遗传研究室，广东省湛江广东医学院附属医院血液内科 |
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| 摘 要： | 测定骨髓转移神经母细胞瘤（ＮＢ）Ｎ－ｍｙｃ扩增情况及评价其与预后的关系。采用差示ＰＣＲ法检测１７例骨髓转移ＮＢ的Ｎ－ｍｙｃ拷贝数，并随访１年。结果，１２例（７０．５９％）证实有Ｎ－ｍｙｃ扩增。Ｎ－ｍｙｃ扩增与ＮＢ分期及患者预后均成显著相关（分别为Ｐ＜０．０５，Ｐ＜０．０１）。有Ｎ－ｍｙｃ扩增者预后差，且扩增倍数越高则预后越差。因此，Ｎ－ｍｙｃ扩增对于ＮＢ分期及预后评估均为有价值的指标，它可能与ｃ－ｍｙｃ协同调控ＮＢ的生物学特性。差示ＰＣＲ作为一种建立在内参照原理上的新方法可定量测定基因拷贝数，比Ｓｏｕｔｈｅｒｎ杂交更简便、快速、灵敏、精确，适于临床应用。尤值一提的是，该法需样品量很少，且无同位素沾染。
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| 关 键 词： | 神经母细胞瘤，癌基因，基因扩增，多聚酶链反应 |
The N-myc Amplification Determination of Neuroblastoma With Bone Marrow Metastasis by Differential Polymerase Chain Reaction |
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| Yang Zhaohui,Zhang Jinhua,Yang Xuezhong ,et al..The N-myc Amplification Determination of Neuroblastoma With Bone Marrow Metastasis by Differential Polymerase Chain Reaction[J].Chinese Journal of Pediatric Surgery,1996(4). |
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| Authors: | Yang Zhaohui Zhang Jinhua Yang Xuezhong |
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| Abstract: | The differential polymerase chain reaction(D-PCR)of 17 cases with myelometastasized Neuroblastoma was measured to determine the numbers of N-myc copy amplification and followed-up for one year.N-myc amplification was confirmed in 12 patients(70.6%)and was correlated with NB staging and patient's outcome(P<0.05 and P<0.01 respectively>.The prognosis of patients with N-myc amplification was poor.The authors demonstrate N-myc amplification is a valuable factor of evaluating the staging and prognosis of NB,it may cooperate with c-mye gene to control the biological characteristics of NB,D-PCR is a new method based on intracontrast principle to determine gene copy quantitatively.Compared with Southern hybridization,it is simple,fast,sensitive,accurate and suitable to clinical use.Furthermore,it needs only a small amount of sample without isotopes contamination. |
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| Keywords: | Neuroblastoma Oncogene Gene amplification Polymerase chain reaction |
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