| 二连浩特口岸羊寄生蜱种类及蜱媒病原调查 |
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| 引用本文： | 曹晓梅,;柳文进,;乔舜,;房魏,;杨鹏飞,;魏怀波,;田丽,;姚李四,;郭天宇,;张晓龙.二连浩特口岸羊寄生蜱种类及蜱媒病原调查[J].中华卫生杀虫药械,2014(5):421-423. |
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| 作者姓名： | 曹晓梅 ;柳文进 ;乔舜 ;房魏 ;杨鹏飞 ;魏怀波 ;田丽 ;姚李四 ;郭天宇 ;张晓龙 |
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| 作者单位： | [1]中国检验检疫科学研究院,北京100123; [2]二连浩特出入境检验检疫局,内蒙古二连浩特011100 |
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| 基金项目： | 国家科技支撑计划课题（编号：2012BAK11B05-02）;科技重大专项（编号：2012Zx10004219-005）. |
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| 摘 要： | 目的调查二连浩特羊寄生蜱种类及蜱携带病原情况。方法对蜱进行形态学种类鉴定,然后提取单只蜱DNA基因组及总RNA,使用PCR技术对莱姆病螺旋体、Q热立克次体、巴贝西原虫、斑点热立克次体、无形体、森林脑炎病毒、布尼亚病毒、克里米亚-刚果出血热病毒等病原体进行检测。结果共采集羊寄生蜱439只,包括亚东璃眼蜱、森林革蜱、草原血蜱。从森林革蜱中检测到斑点热立克次体1例,检出率为0.227 8%,其他病原未检出。结论在二连浩特口岸地区蜱种中存在斑点热立克次体感染。
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| 关 键 词： | 二连浩特 蜱 病原 斑点热立克次体 |
Survey for the tick species parasitized in sheep and the tick-borne pathogeny in Erenhot |
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| Institution: | CAO Xiao-mei , LIU Wen-jin , QIAO Shun, FANG Wei , YANG Peng-fei , WEI Huai-bo , TIAN Li , YAO Li-si , GUO Tian-yu ,ZHANG Xiao-long (1. Chinese Academy of Inspection and Quarantine Science Research ,Beijing 100123, China; 2. Erlianhot Entry-exit Inspection and Quarantine Bureau, Erlianhot 011100, China) |
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| Abstract: | Objective To investigate the pathogeny carried by the tick which parasitize sheep in Erenhot. Meth- ods The genome and total RNA were extracted from single tick respectively after the species identification. Borrelia burgdorferi, Q fever rickettsia, Babesia microti, SFGR, HGA , russian spring-summer encephalitis virus , bunyavirus and CCHF were detected by PCR and RT - PCR technology. Results Total 439 sheep parasitic ticks were collect- ed, including Hyalomma asiaticum ,Dermacentor silvarum and Haemaphysalis concinne. One case of SFGR was detected from the body of Dermacentor silvarum with detection rates of 0.227 8 %, but the other seven species of pathogen were not detected by PCR. Conclusion There is spotted fever rickettsial infections in population of tick in Erenhot port regiion. |
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| Keywords: | Erenhot ticks pathogeny spotted fever rickettsial |
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