| 2.2.15细胞中HBV DNA前C和C区PCR扩增及其产物测序 |
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| 引用本文： | 徐华模,张定凤.2.2.15细胞中HBV DNA前C和C区PCR扩增及其产物测序[J].泸州医学院学报,1995,18(3):166-168. |
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| 作者姓名： | 徐华模 张定凤 |
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| 作者单位： | 泸州医学院附属医院传染科!646000(徐华模，钟森，唐坤华)，重庆医科大学肝炎研究所(张定凤) |
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| 摘 要： | 应用聚合酶链反应（ＰＣＲ）及其扩增产物的直接测序技术对２．２．１５细胞中乙型肝炎病毒（ＨＢＶ）基因前Ｃ和Ｃ区进行测序。结果表明，根据ａｙｗ亚型ＨＢＶ基因设计的引物能够扩增２．２．１５细胞中ＨＢＶＤＮＡ扩增产物位于２２１－２９８碱基对之间，而对ａｄｒ亚型ＨＢＶＤＮＡ无扩增作用，说明ＰＣＲ扩增是特异性的，所测到的１３２个碱基序列与ａｗｙ１亚型ＨＢＶＤＮＡ完全符合，提示２．２．１５细胞中ＨＢＶＤＮＡ属ａ
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| 关 键 词： | 乙型肝炎病毒 细胞培养 脱氧核糖核酸 测序 |
AMPLIFICATION OF POLYMERASE CHAIN REACTION AND DIRECT SEQUENCING OF HEPATITS B VIRAL GENOME ENCOMPASSING PRE-C AND C REGION IN 2. 2. 15 CELLS |
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| Xu Huamu et al.AMPLIFICATION OF POLYMERASE CHAIN REACTION AND DIRECT SEQUENCING OF HEPATITS B VIRAL GENOME ENCOMPASSING PRE-C AND C REGION IN 2. 2. 15 CELLS[J].Journal of Luzhou Medical College,1995,18(3):166-168. |
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| Authors: | Xu Huamu |
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| Institution: | Luzlou Medical W Hosptial |
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| Abstract: | The sequence of hepatitis B viral genome in the 2. 2. 15 cells was determined amplification of polymerase chain reaction (PCR) and direct sequencing of its amplified products. Our resultsshowed that PCR amplification was specifical and HBV DNA in the 2. 2. 15 cells was from HBV withsurface antigen subtype aywl,because amplified products were located between bp 221 and bp 298 andthe sequence was in accord with subtype aywl. We first identifid the subtype of HBV genome in the2- 2. 15 cells, which might play an improtant role in researches on anti-HBV drugs in vitro cell culture assay system. |
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| Keywords: | Hepatitis B virus Polymerase chain reaction Sequencing Human hepatoblastoma cell lines |
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