| LY294002联合AD-PTEN对脑胶质瘤细胞增殖和侵袭的影响及机制探讨 |
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| 引用本文： | 宋云朋,钟跃,刘哲,潘强.LY294002联合AD-PTEN对脑胶质瘤细胞增殖和侵袭的影响及机制探讨[J].山东医药,2010,50(2):1-3. |
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| 作者姓名： | 宋云朋 钟跃 刘哲 潘强 |
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| 作者单位： | 天津医科大学总医院,天津,300052 |
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| 基金项目： | 天津市科委应用基础计划 |
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| 摘 要： | 目的观察P13K抑制剂LY294002联合含VIEN基因的重组腺病毒（Ad—PTEN）对人脑胶质瘤TJ899细胞增殖和侵袭的影响，并探讨其机制。方法向TJ889分别加入LY294002（LY294002组）、Ad-PTEN＋LY294002（联合组）、Ad—vector病毒（空载组）、DMSO（DMSO组）培养48h。分别采用MTY法、流式细胞术、划痕实验和Tran．swell小室观察TJ899增殖活性和侵袭能力的变化；Western Blot检测TJ899的PTEN／P13K／AKT信号转导通路中相关蛋白。结果与DMSO组、空载组和LY294002组相比，联合组细胞存活速率下降，细胞周期阻滞在G0／G1期，S期减少，细胞凋亡率增加，侵袭和迁移的细胞数减少，PTEN蛋白表达增加，而磷酸化蛋白激酶B、细胞增殖抗原、细胞周期素D1、Bcl-2、基质金属蛋白酶-2、黏着斑激酶蛋白表达显著下降，P均〈0．05。结论LY294002联合Ad-PTEN可明显抑制TJ899的增殖活性和侵袭能力，该作用与抑制P13K／AKT信号通路有关。
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| 关 键 词： | 颅内肿瘤 胶质瘤 酪氨酸磷酸酶基因 P13K抑制剂 LY294002 信号通路 |
Inhibition effect and mechanism of LY294002 and Ad-PTEN on proliferation and invasion activity in glioma cell line TJ889 cell |
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| SONG Yun-peng,ZHONG Yue,LIU zhe,PAN Qiang.Inhibition effect and mechanism of LY294002 and Ad-PTEN on proliferation and invasion activity in glioma cell line TJ889 cell[J].Shandong Medical Journal,2010,50(2):1-3. |
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| Authors: | SONG Yun-peng ZHONG Yue LIU zhe PAN Qiang |
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| Institution: | SONG Yun-peng,ZHONG Yue,LIU zhe,PAN Qiang(General Hospital,Tianjin Medical University,Tianjin300052,P.R.China) |
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| Abstract: | Objective To observe the inhibition effect and mechanism of LY294002 and Ad-PTEN on proliferation and invasion in glioma cell line TJ889 cell.Methods Ad-PTEN were transfected into TJ889,being treated by LY294002.The proliferation and invasion activity of TJ889 cells was examined by MTT,Flow Cytometry,Annexin V methods,Transwell and scarification test respectively.Western blot analysis was used to detect the protein expression in PI3K/AKT signal pathway.Results The cell multiplication rate in combination gro... |
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| Keywords: | LY294002 |
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