通络方剂减轻糖尿病大鼠肾小球足细胞损伤

目的：采用糖尿病大鼠模型研究通络方剂对肾小球足细胞的保护作用及其可能的机制。 方法：SD大鼠分为正常对照组、糖尿病对照组、通络方剂组和α硫辛酸组。干预组大鼠分别予以通络方剂（0.4g/kg/d）、α硫辛酸（100mg/kg/d）灌胃，干预12周后处死大鼠，留取血清和尿标本，测定肾重体重比，24h尿蛋白定量、BUN、Cr、Alb/Cr等肾功能指标，留取肾组织标本，体视学方法计数足细胞数量，计算足细胞相对密度，western blot方法检测肾皮质Nephrin的表达，同时测定肾皮质MDA含量和SOD活力。 结果：通络方剂组和α硫辛酸组大鼠24小时尿蛋白定量低于DM对照组，足细胞计数和Nephrin表达量高于DM对照组，差异有统计学意义（p<0.05）；与DM组相比，通络方剂组和α硫辛酸组大鼠肾皮质MDA含量较少，SOD活力较高（p<0.05）。 结论：通络方剂对糖尿病大鼠肾小球足细胞损伤具有保护作用，其机制可能与其抗氧化作用有关。

Protective effects of Tongluo recipe against injury of glomerular visceral epithelial cells in diabetic rats

Objective To observe the protective effect of α-lipoic acid(ALA) and Tongluo recipe(TLR) against injury of glomerular visceral epithelial cells in diabetic rats,so as to discuss the possible mechanism for injury of glomerular visceral epithelial cells in diabetes.Methods Rat diabetes model was induced by high-fat diet and a single intraperitoneal injection of streptozotocin(35 mg/kg).The diabetic model rats were randomly divided into three groups(n=8): diabetes mellitus(DM) group,DM+TLR and DM+ALA group;another 8 rats served as normal controls(CON group).Rats in the DM+TLR group received 0.4 g·kg-1·d-1 TLR and DM+ALA group received 0.1 g·kg-1·d-1 ALA throughout the experiment.Rats in the DM group and control(CON) group were given celiac perfusion of distilled water once at the same time and volume.After 12 weeks of treatment,kidney mass/body mass index,24 h urinary protein,blood urea nitrogen,and urinary albumin/Cr were determined in each group.The number and the density of podocytes in glomerulis were measured by stereological dissector/fractionator methods.The expression of nephrin in renal cortical tissue was detected by Western blotting analysis.The activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and the content of malondialdehyde(MDA)were measured in the plasma and renal cortical tissues.Results After 12 weeks,the kidney mass/body mass index,24 h urinary protein and urinary albumin/Cr in DM group were significantly increased compared to control group(P<0.01);the number and density of podocytes in the glomerulis and nephrin expression in DM group were significantly decreased(P<0.01).The kidney mass/body mass index,24 h urinary protein and urinary albumin/Cr in DM+TLR and DM+ALA groups were significantly decreased compared to DM group(P<0.01);the number and density of podocytes and nephrin expression in DM+TLR and DM+ALA groups were significantly increased compared to DM group(P<0.01,P<0.05).GSH-Px and SOD activities were significantly decreased in the plasma(P<0.01) and renal cortical tissue(P<0.01) in DM,DM+ALA and DM+TLR groups compared to those in the CON group,and the content of MDA was significantly increased(P<0.01,P<0.05).GSH-Px and SOD activities were significantly increased in the plasma and renal cortical tissue(P both<0.01) and MDA content was significantly decreased in DM+TLR and DM+ALA groups(P<0.01,P<0.05)compared to those in the DM group.No significant differences were found between DM+ALA group and DM+TLR group concerning the above parameters.Conclusion TLR can ameliorate podocyte injury in diabetic rats,which might be associated with its anti-oxidative effect.