| 淫羊藿苷对兔阴蒂海绵体平滑肌细胞NO及NOS活性的影响 |
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| 引用本文： | 杨春,辛钟成,付杰,袁亦铭,丁义,辛华,汪泽厚.淫羊藿苷对兔阴蒂海绵体平滑肌细胞NO及NOS活性的影响[J].中国男科学杂志,2005,19(1):6-10. |
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| 作者姓名： | 杨春 辛钟成 付杰 袁亦铭 丁义 辛华 汪泽厚 |
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| 作者单位： | 1. 北京大学第一医院泌尿外科,北京大学泌尿外科研究所,北京,100034
2. 北京空军总医院泌尿外科 |
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| 基金项目： | 北京市自然科学基金(7012023) |
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| 摘 要： | 目的 探讨淫羊藿苷对体外培养的兔阴蒂海绵体平滑肌细胞一氧化氮(NO)产生及一氧化氮合酶(NOS)活性的作用效果。 方法 取兔阴蒂海绵体采用酶消化法进行平滑肌细胞体外培养,利用免疫细胞化学染色法通过检测α-actin进行细胞鉴定;利用硝酸还原酶法及NOS试剂盒测定不同浓度淫羊藿苷对阴蒂海绵体平滑肌细胞NO生成及NOS活性的影响。 结果 培养的兔阴蒂海绵体平滑肌细胞呈现典型的平滑肌细胞形态特征;淫羊藿苷浓度依赖性增强家兔阴蒂海绵体平滑肌细胞NOS活性并增加NO生成(P<0.01),且被NOS抑制剂L-硝基精氨酸(LNNA)所抑制(P<0.01)。结论 淫羊藿苷可能通过增强NOS活性而提高阴蒂海绵体平滑肌细胞NO生成,增强性刺激下阴蒂海绵体平滑肌的松弛作用而增强阴蒂胀大勃起功能。
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| 关 键 词： | 淫羊藿苷 阴蒂海绵体平滑肌细胞 一氧化氮合酶 |
| 修稿时间： | 2004年5月10日 |
Effects of Icariin on NO production and NOS activity of rabbit clitoral corporal smooth muscle cells |
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| Yang Chun,Xin Zhongcheng,Fu Jie,Yuan Yiming,Ding Yi,Xin Hua,Wang ZehouInstitute of Urology,Peking University.Effects of Icariin on NO production and NOS activity of rabbit clitoral corporal smooth muscle cells[J].Chinese Journal of Andrology,2005,19(1):6-10. |
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| Authors: | Yang Chun Xin Zhongcheng Fu Jie Yuan Yiming Ding Yi Xin Hua Wang ZehouInstitute of Urology Peking University |
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| Institution: | Yang Chun,Xin Zhongcheng,Fu Jie,Yuan Yiming,Ding Yi,Xin Hua,Wang ZehouInstitute of Urology,Peking University,Department of Urology,First Hospital,Peking University,Beijing 100034,China |
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| Abstract: | Objective To study the effects of Icariin on nitric oxide(NO) production and nitric oxide synthase(NOS) activity in rabbit clitoral corporal smooth muscle cells(RCSMC). Methods In vitro the rabbit clitoral SMCwere cultured by enzymatically dispersed method; the effects of Icarrin on NO production and NOS activity inRCSMC were measured by nitrate reductase test and NOS kit. Results Cultured clitoral cells exhibited typicalmorphologic characteristics of SMC; NO production and NOS activity in RCSMC treated by Icariin were increasedsignificantly in a dose dependent manner(P<0.01). Therefore, these responses were significantly inhibited by NOSinhibitor LNNA(P<0.01). Conclusions Icariin may increase NO production by activating NOS in clitoral SMC, andmay be effective for clitoral corporal smooth muscle relaxation inducing clitoris tumescence during sexual stimulation. |
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| Keywords: | Icariin clitoral corporal smooth muscle cell NOS |
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