Alloxan-glucose interaction: Effect on incorporation of14C-leucine into pancreatic islets of rat |
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Authors: | Renu Bansal Nafees Ahmad Jalil R Kidwai |
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Institution: | (1) Division of Biochemistry, Central Drug Research Institute, 226001 Lucknow, India |
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Abstract: | Summary The acute effect of alloxan on the incorporation of14C-leucine into isolated rat islets of Langerhans was studied. I.v. administration of alloxan (40 mg/kg body weight) in rats
inhibited the subsequentin vitro incorporation of14C-leucine into (pro-)insulin in the isolated islets. Glucose (750 mg/kg body weight), when administered 5 min prior to alloxan,
completely protected the islets against alloxan toxicity. The protective effect of glucose was partly reversed when the concentration
of alloxan was raised to 80 mg/kg body weight. Similar results of inhibition of (pro-)insulin biosynthesis by alloxan and
its protection by glucose were obtained when isolated rat islets were exposed to alloxan and/or glucosein vitro. Islets exposed to glucosein vitro immediately after alloxan exposure showed a slower rate of inhibition of (pro-)insulin biosynthesis, as compared to islets
washed before exposure to D-glucose. In view of these findings, it is suggested that there is a common recognition site on
B-cell for alloxan and glucose.
CDRI Communication No. 2617. |
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Keywords: | Alloxan D-glucose Islets of Langerhans Pancreas (Pro-) insulin |
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